Source:http://linkedlifedata.com/resource/pubmed/id/15656577
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
1
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| pubmed:dateCreated |
2005-1-19
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| pubmed:abstractText |
Human estrogen receptor-positive breast cancer cells typically display elevated levels of Myc protein due to overexpression of MYC mRNA, and elevated insulin-like growth factor 1 receptor (IGF1R) due to overexpression of IGF1R mRNA. We hypothesized that scintigraphic detection of MYC peptide nucleic acid (PNA) probes with an IGF1 peptide loop on the C-terminus, and a [99mTc]chelator peptide on the N-terminus, could measure levels of MYC mRNA noninvasively in human IGF1R-overexpressing MCF7 breast cancer xenografts in nude mice. We prepared the chelator-MYC PNA-IGF1 peptide, as well as a 4-nt mismatch PNA control, by solid-phase synthesis. We imaged MCF7 xenografts scintigraphically and measured the distribution of [99mTc]probes by scintillation counting of dissected tissues. MCF7 xenografts in nude mice were visualized at 4 and 24 h after tail vein administration of the [99mTc]PNA probe specific for MYC mRNA, but not with the mismatch control. The [99mTc]probes distributed normally to the kidneys, livers, tumors, and other tissues. Molecular imaging of oncogene mRNAs in solid tumors with radiolabel-PNA-peptide chimeras might provide additional genetic characterization of preinvasive and invasive breast cancers.
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| pubmed:grant | |
| pubmed:language |
eng
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| pubmed:journal | |
| pubmed:citationSubset |
IM
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| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Peptide Nucleic Acids,
http://linkedlifedata.com/resource/pubmed/chemical/Proto-Oncogene Proteins c-myc,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Messenger,
http://linkedlifedata.com/resource/pubmed/chemical/Receptor, IGF Type 1,
http://linkedlifedata.com/resource/pubmed/chemical/Technetium Tc 99m Sestamibi
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| pubmed:status |
MEDLINE
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| pubmed:issn |
1043-1802
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| pubmed:author | |
| pubmed:issnType |
Print
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| pubmed:volume |
16
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| pubmed:owner |
NLM
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| pubmed:authorsComplete |
Y
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| pubmed:pagination |
70-9
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| pubmed:dateRevised |
2009-11-19
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| pubmed:meshHeading |
pubmed-meshheading:15656577-Animals,
pubmed-meshheading:15656577-Breast Neoplasms,
pubmed-meshheading:15656577-Chimera,
pubmed-meshheading:15656577-Diagnostic Imaging,
pubmed-meshheading:15656577-Disease Models, Animal,
pubmed-meshheading:15656577-Female,
pubmed-meshheading:15656577-Gene Expression Regulation, Neoplastic,
pubmed-meshheading:15656577-Humans,
pubmed-meshheading:15656577-Mice,
pubmed-meshheading:15656577-Mice, Nude,
pubmed-meshheading:15656577-Neoplasm Transplantation,
pubmed-meshheading:15656577-Peptide Nucleic Acids,
pubmed-meshheading:15656577-Proto-Oncogene Proteins c-myc,
pubmed-meshheading:15656577-RNA, Messenger,
pubmed-meshheading:15656577-Receptor, IGF Type 1,
pubmed-meshheading:15656577-Technetium Tc 99m Sestamibi,
pubmed-meshheading:15656577-Time Factors,
pubmed-meshheading:15656577-Tissue Distribution,
pubmed-meshheading:15656577-Tumor Cells, Cultured,
pubmed-meshheading:15656577-Xenograft Model Antitumor Assays
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| pubmed:articleTitle |
Noninvasive molecular imaging of MYC mRNA expression in human breast cancer xenografts with a [99mTc]peptide-peptide nucleic acid-peptide chimera.
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| pubmed:affiliation |
Department of Biochemistry and Molecular Pharmacology, Thomas Jefferson University, Philadelphia, Pennsylvania 19107, USA.
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| pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, U.S. Gov't, Non-P.H.S.,
Research Support, N.I.H., Extramural
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