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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
8
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pubmed:dateCreated |
1992-5-15
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pubmed:abstractText |
The two subunits of the human class I histocompatibility antigen (HLA)-A2 have been expressed at high levels (20-30 mg/liter) as insoluble aggregates in bacterial cells. The aggregates were dissolved in 8 M urea and then refolded to form an HLA-A2-peptide complex by removal of urea in the presence of an antigenic peptide. Two peptides from the matrix protein and nucleoprotein of influenza virus are known to bind to HLA-A2, and both support the refolding of the recombinant HLA-A2 molecule. An additional peptide, a nonamer from the gp120 envelope protein of human immunodeficiency virus type 1, also supported refolding. Yields of purified recombinant HLA-A2 are 10-15%. In the absence of an HLA-A2-restricted peptide, a stable HLA-A2 complex was not formed. Monoclonal antibodies known to bind to native HLA-A2 also bound to the recombinant HLA-A2-peptide complex. Three purified HLA-A2-peptide complexes refolded from bacterially produced protein aggregates crystallize under the identical conditions as HLA-A2 purified from human lymphoblastoid cells. Crystals of the recombinant HLA-A2 molecule in complex with the influenza matrix nonamer peptide, Mp(58-66), diffract to greater than 1.5-A resolution.
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pubmed:grant |
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/1565634-1369317,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1565634-1553383,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1565634-1709722,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1565634-1717555,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1565634-1721637,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1565634-1922337,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1565634-1922338,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1565634-2017257,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1565634-2038058,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1565634-2367533,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1565634-2408046,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1565634-2437457,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1565634-2443855,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1565634-2451830,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1565634-2469442,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1565634-2480387,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1565634-2594067,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1565634-2687112,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1565634-3309677,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1565634-3323806,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1565634-3760563,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1565634-3878413,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1565634-4129801,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1565634-4514327,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1565634-6189821,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1565634-6190942,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1565634-667938,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1565634-6982419,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1565634-714164,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1565634-72068,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1565634-781677
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Antibodies, Monoclonal,
http://linkedlifedata.com/resource/pubmed/chemical/HIV Envelope Protein gp120,
http://linkedlifedata.com/resource/pubmed/chemical/HLA-A2 Antigen,
http://linkedlifedata.com/resource/pubmed/chemical/Macromolecular Substances,
http://linkedlifedata.com/resource/pubmed/chemical/Nucleoproteins,
http://linkedlifedata.com/resource/pubmed/chemical/Oligodeoxyribonucleotides,
http://linkedlifedata.com/resource/pubmed/chemical/Peptides,
http://linkedlifedata.com/resource/pubmed/chemical/Recombinant Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Viral Matrix Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Viral Proteins
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pubmed:status |
MEDLINE
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pubmed:month |
Apr
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pubmed:issn |
0027-8424
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
15
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pubmed:volume |
89
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
3429-33
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pubmed:dateRevised |
2010-9-7
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pubmed:meshHeading |
pubmed-meshheading:1565634-Amino Acid Sequence,
pubmed-meshheading:1565634-Antibodies, Monoclonal,
pubmed-meshheading:1565634-Base Sequence,
pubmed-meshheading:1565634-Cloning, Molecular,
pubmed-meshheading:1565634-Crystallization,
pubmed-meshheading:1565634-Escherichia coli,
pubmed-meshheading:1565634-HIV Envelope Protein gp120,
pubmed-meshheading:1565634-HLA-A2 Antigen,
pubmed-meshheading:1565634-Humans,
pubmed-meshheading:1565634-Macromolecular Substances,
pubmed-meshheading:1565634-Molecular Sequence Data,
pubmed-meshheading:1565634-Nucleoproteins,
pubmed-meshheading:1565634-Oligodeoxyribonucleotides,
pubmed-meshheading:1565634-Peptides,
pubmed-meshheading:1565634-Plasmids,
pubmed-meshheading:1565634-Polymerase Chain Reaction,
pubmed-meshheading:1565634-Protein Binding,
pubmed-meshheading:1565634-Protein Conformation,
pubmed-meshheading:1565634-Protein Denaturation,
pubmed-meshheading:1565634-Recombinant Proteins,
pubmed-meshheading:1565634-Restriction Mapping,
pubmed-meshheading:1565634-Viral Matrix Proteins,
pubmed-meshheading:1565634-Viral Proteins
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pubmed:year |
1992
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pubmed:articleTitle |
HLA-A2-peptide complexes: refolding and crystallization of molecules expressed in Escherichia coli and complexed with single antigenic peptides.
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pubmed:affiliation |
Department of Biochemistry and Molecular Biology, Howard Hughes Medical Institute, Harvard University, Cambridge, MA 02138-2092.
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pubmed:publicationType |
Journal Article
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