Switch to
| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
2
|
| pubmed:dateCreated |
1977-6-11
|
| pubmed:abstractText |
Some properties of mouse spleen cytosol inorgainc pyrophosphatase (PPi-ase) (E. C. 3.6.1.1) as well as the effect of methylene diphosphonic acid (PCP) on the PPi-ase activity were studied. Specific staining for the enzyme PAAG disc-electrophoresis was developed; it was shown that the PPi-ase formed only one band in 7.5% PAAG. The enzyme pH optimum being 8.0, the optimal [Mg++]/[PPi] ratio was about 2; Km =7.7x10(-4) M, Vmax=0.77 mkM. min-1. mg protein-1. PCP was shown to competitively inhibit the pyrophosphatase reaction, Ki=2.5x10(-4) M +/- 0.2x10(-4) M.
|
| pubmed:language |
rus
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
Feb
|
| pubmed:issn |
0320-9725
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
42
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
238-42
|
| pubmed:dateRevised |
2007-7-23
|
| pubmed:meshHeading |
pubmed-meshheading:15654-Animals,
pubmed-meshheading:15654-Binding, Competitive,
pubmed-meshheading:15654-Cytosol,
pubmed-meshheading:15654-Diphosphonates,
pubmed-meshheading:15654-Hydrogen-Ion Concentration,
pubmed-meshheading:15654-Magnesium,
pubmed-meshheading:15654-Mice,
pubmed-meshheading:15654-Pyrophosphatases,
pubmed-meshheading:15654-Spleen
|
| pubmed:year |
1977
|
| pubmed:articleTitle |
[Inhibition of mouse spleen inorganic pyrophosphatase by methylene diphosphonic acid].
|
| pubmed:publicationType |
Journal Article,
English Abstract
|