Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
2005-1-18
pubmed:abstractText
Progress in the field of neurogenesis is currently limited by the lack of tools enabling fast and quantitative analysis of neurogenesis in the adult brain. Doublecortin (DCX) has recently been used as a marker for neurogenesis. However, it was not clear whether DCX could be used to assess modulations occurring in the rate of neurogenesis in the adult mammalian central nervous system following lesioning or stimulatory factors. Using two paradigms increasing neurogenesis levels (physical activity and epileptic seizures), we demonstrate that quantification of DCX-expressing cells allows for an accurate measurement of modulations in the rate of adult neurogenesis. Importantly, we excluded induction of DCX expression during physiological or reactive gliogenesis and excluded also DCX re-expression during regenerative axonal growth. Our data validate DCX as a reliable and specific marker that reflects levels of adult neurogenesis and its modulation. We demonstrate that DCX is a valuable alternative to techniques currently used to measure the levels of neurogenesis. Importantly, in contrast to conventional techniques, analysis of neurogenesis through the detection of DCX does not require in vivo labelling of proliferating cells, thereby opening new avenues for the study of human neurogenesis under normal and pathological conditions.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
http://linkedlifedata.com/resource/pubmed/chemical/Bromodeoxyuridine, http://linkedlifedata.com/resource/pubmed/chemical/DAPI, http://linkedlifedata.com/resource/pubmed/chemical/GAP-43 Protein, http://linkedlifedata.com/resource/pubmed/chemical/Glial Fibrillary Acidic Protein, http://linkedlifedata.com/resource/pubmed/chemical/Indoles, http://linkedlifedata.com/resource/pubmed/chemical/Microtubule-Associated Proteins, http://linkedlifedata.com/resource/pubmed/chemical/Neurofilament Proteins, http://linkedlifedata.com/resource/pubmed/chemical/Neuropeptides, http://linkedlifedata.com/resource/pubmed/chemical/Phosphopyruvate Hydratase, http://linkedlifedata.com/resource/pubmed/chemical/Scopolamine Hydrobromide, http://linkedlifedata.com/resource/pubmed/chemical/doublecortin protein, http://linkedlifedata.com/resource/pubmed/chemical/neurofilament protein H
pubmed:status
MEDLINE
pubmed:month
Jan
pubmed:issn
0953-816X
pubmed:author
pubmed:issnType
Print
pubmed:volume
21
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1-14
pubmed:dateRevised
2011-11-17
pubmed:meshHeading
pubmed-meshheading:15654838-Animals, pubmed-meshheading:15654838-Animals, Newborn, pubmed-meshheading:15654838-Behavior, Animal, pubmed-meshheading:15654838-Brain, pubmed-meshheading:15654838-Bromodeoxyuridine, pubmed-meshheading:15654838-Cell Count, pubmed-meshheading:15654838-Cell Differentiation, pubmed-meshheading:15654838-Cell Proliferation, pubmed-meshheading:15654838-Cell Size, pubmed-meshheading:15654838-Cells, Cultured, pubmed-meshheading:15654838-Female, pubmed-meshheading:15654838-GAP-43 Protein, pubmed-meshheading:15654838-Ganglia, Spinal, pubmed-meshheading:15654838-Gene Expression Regulation, pubmed-meshheading:15654838-Glial Fibrillary Acidic Protein, pubmed-meshheading:15654838-Immunohistochemistry, pubmed-meshheading:15654838-Indoles, pubmed-meshheading:15654838-Laminectomy, pubmed-meshheading:15654838-Mice, pubmed-meshheading:15654838-Mice, Inbred C57BL, pubmed-meshheading:15654838-Microtubule-Associated Proteins, pubmed-meshheading:15654838-Neurofilament Proteins, pubmed-meshheading:15654838-Neurons, pubmed-meshheading:15654838-Neuropeptides, pubmed-meshheading:15654838-Organ Culture Techniques, pubmed-meshheading:15654838-Phosphopyruvate Hydratase, pubmed-meshheading:15654838-Running, pubmed-meshheading:15654838-Scopolamine Hydrobromide, pubmed-meshheading:15654838-Seizures, pubmed-meshheading:15654838-Spinal Cord Injuries, pubmed-meshheading:15654838-Stem Cells, pubmed-meshheading:15654838-Time Factors
pubmed:year
2005
pubmed:articleTitle
Doublecortin expression levels in adult brain reflect neurogenesis.
pubmed:affiliation
Volkswagen-Foundation Junior Group, University of Regensburg, Franz-Josef-Strauss Allee 11, 93053 Regensburg, Germany.
pubmed:publicationType
Journal Article, Comparative Study, Research Support, Non-U.S. Gov't