Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
4
pubmed:dateCreated
2005-3-28
pubmed:abstractText
Angularly resolved light scattering measurements were performed on suspensions of EMT6 cells and on mitochondria isolated from rabbit liver. Mie theory analysis of the scattering from intact cells indicated that mitochondrial-sized organelles dominated scattering in the range 5-90 degrees . This interpretation was supported by the analysis of scattering from isolated mitochondria. Intact cells were subjected to oxidative stress by photodynamic insult. After 3 h of incubation in the heme precursor aminolevulinic acid hexylester, EMT6 cells accumulated abundant protoporphyrin IX, an endogenous photosensitizer formed in mitochondria. Irradiation of aminolevulinic acid/protoporphyrin IX-sensitized cells with 10 J cm(-2) of 514 nm light led to pronounced changes in angularly resolved light scattering consistent with mitochondrial swelling. Electron microscopy of similarly treated EMT6 cell monolayers showed significant changes in mitochondrial morphology, which included distension of the outer unit membrane and bloating of the internal mitochondrial compartment. Informed by these electron microscopy results, we implemented a coated sphere model to interpret the scattering from intact cells subjected to oxidative stress. The coated sphere interpretation was compatible with the scattering measurements from these cells, whereas simpler Mie theory models based on homogenous swelling were dramatically unsuccessful. Thus, in this system, angularly resolved light scattering reports oxidative-stress-induced changes in mitochondrial morphology.
pubmed:grant
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/15653724-10200545, http://linkedlifedata.com/resource/pubmed/commentcorrection/15653724-11579101, http://linkedlifedata.com/resource/pubmed/commentcorrection/15653724-11916880, http://linkedlifedata.com/resource/pubmed/commentcorrection/15653724-12175287, http://linkedlifedata.com/resource/pubmed/commentcorrection/15653724-12202392, http://linkedlifedata.com/resource/pubmed/commentcorrection/15653724-12659143, http://linkedlifedata.com/resource/pubmed/commentcorrection/15653724-13069686, http://linkedlifedata.com/resource/pubmed/commentcorrection/15653724-14429695, http://linkedlifedata.com/resource/pubmed/commentcorrection/15653724-15008533, http://linkedlifedata.com/resource/pubmed/commentcorrection/15653724-15377529, http://linkedlifedata.com/resource/pubmed/commentcorrection/15653724-1557830, http://linkedlifedata.com/resource/pubmed/commentcorrection/15653724-167945, http://linkedlifedata.com/resource/pubmed/commentcorrection/15653724-4134589, http://linkedlifedata.com/resource/pubmed/commentcorrection/15653724-5968972, http://linkedlifedata.com/resource/pubmed/commentcorrection/15653724-7785769, http://linkedlifedata.com/resource/pubmed/commentcorrection/15653724-8778819, http://linkedlifedata.com/resource/pubmed/commentcorrection/15653724-9066303, http://linkedlifedata.com/resource/pubmed/commentcorrection/15653724-9721092
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Apr
pubmed:issn
0006-3495
pubmed:author
pubmed:issnType
Print
pubmed:volume
88
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
2929-38
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
pubmed-meshheading:15653724-Aminolevulinic Acid, pubmed-meshheading:15653724-Animals, pubmed-meshheading:15653724-Biophysics, pubmed-meshheading:15653724-Cell Line, Tumor, pubmed-meshheading:15653724-Culture Techniques, pubmed-meshheading:15653724-Light, pubmed-meshheading:15653724-Liver, pubmed-meshheading:15653724-Mice, pubmed-meshheading:15653724-Microscopy, Electron, pubmed-meshheading:15653724-Microscopy, Polarization, pubmed-meshheading:15653724-Mitochondria, pubmed-meshheading:15653724-Mitochondrial Swelling, pubmed-meshheading:15653724-Models, Statistical, pubmed-meshheading:15653724-Oxidative Stress, pubmed-meshheading:15653724-Oxygen Consumption, pubmed-meshheading:15653724-Protoporphyrins, pubmed-meshheading:15653724-Rabbits, pubmed-meshheading:15653724-Scattering, Radiation, pubmed-meshheading:15653724-Time Factors
pubmed:year
2005
pubmed:articleTitle
Light scattering from intact cells reports oxidative-stress-induced mitochondrial swelling.
pubmed:affiliation
Department of Physics and Astronomy, University of Rochester, Rochester, New York 14642, USA.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, N.I.H., Extramural