|
rdf:type |
|
|
lifeskim:mentions |
|
|
pubmed:issue |
2
|
|
pubmed:dateCreated |
2005-1-14
|
|
pubmed:abstractText |
It has been reported that diabetes-induced inappropriate apoptosis in embryos during neurulation may be one of the mechanisms leading to neural tube defects. We studied apoptosis and the apoptotic pathway occurring in early post-implantation period embryos of non-diabetic and streptozotocin (STZ)-induced diabetic rats. In quantitative RT-PCR, bax mRNA was constantly expressed to similar degree in embryos of non-diabetic and diabetic rats, while the expression of bcl-2 mRNA was significantly decreased in diabetic rat embryos compared to non-diabetic rat embryos. The increased number of terminal deoxynucleotidyl transferase-mediated nick end labeling (TUNEL)-positive cells occurred selectively in the primitive brains of diabetic rat embryos compared to non-diabetic rat embryos. Immunohistochemical studies revealed that, in mirror sections, the staining of Bax and activated caspase-3 were observed in the TUNEL-positive cell area, but the expression of Bcl-2 in these apoptotic cells was generally too low to be detected. These results suggest that a Bax-regulated mitochondrial cytochrome c-mediated caspase-3 activation pathway might be involved in the diabetic embryopathy.
|
|
pubmed:language |
eng
|
|
pubmed:journal |
|
|
pubmed:citationSubset |
IM
|
|
pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Bax protein, rat,
http://linkedlifedata.com/resource/pubmed/chemical/Casp3 protein, rat,
http://linkedlifedata.com/resource/pubmed/chemical/Caspase 3,
http://linkedlifedata.com/resource/pubmed/chemical/Caspases,
http://linkedlifedata.com/resource/pubmed/chemical/Cytochromes c,
http://linkedlifedata.com/resource/pubmed/chemical/Proto-Oncogene Proteins c-bcl-2,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Messenger,
http://linkedlifedata.com/resource/pubmed/chemical/bcl-2-Associated X Protein
|
|
pubmed:status |
MEDLINE
|
|
pubmed:month |
Feb
|
|
pubmed:issn |
0168-8227
|
|
pubmed:author |
|
|
pubmed:issnType |
Print
|
|
pubmed:volume |
67
|
|
pubmed:owner |
NLM
|
|
pubmed:authorsComplete |
Y
|
|
pubmed:pagination |
110-8
|
|
pubmed:dateRevised |
2007-11-15
|
|
pubmed:meshHeading |
pubmed-meshheading:15649569-Animals,
pubmed-meshheading:15649569-Apoptosis,
pubmed-meshheading:15649569-Caspase 3,
pubmed-meshheading:15649569-Caspases,
pubmed-meshheading:15649569-Cytochromes c,
pubmed-meshheading:15649569-Diabetes Mellitus, Experimental,
pubmed-meshheading:15649569-Embryo, Mammalian,
pubmed-meshheading:15649569-Embryonic Development,
pubmed-meshheading:15649569-Female,
pubmed-meshheading:15649569-Genes, bcl-2,
pubmed-meshheading:15649569-Immunohistochemistry,
pubmed-meshheading:15649569-In Situ Nick-End Labeling,
pubmed-meshheading:15649569-Pregnancy,
pubmed-meshheading:15649569-Pregnancy in Diabetics,
pubmed-meshheading:15649569-Proto-Oncogene Proteins c-bcl-2,
pubmed-meshheading:15649569-RNA, Messenger,
pubmed-meshheading:15649569-Rats,
pubmed-meshheading:15649569-Rats, Wistar,
pubmed-meshheading:15649569-Reverse Transcriptase Polymerase Chain Reaction,
pubmed-meshheading:15649569-bcl-2-Associated X Protein
|
|
pubmed:year |
2005
|
|
pubmed:articleTitle |
Apoptosis and its pathway in early post-implantation embryos of diabetic rats.
|
|
pubmed:affiliation |
Department of Metabolism/Diabetes and Clinical Nutrition, Nagasaki University Hospital of Medicine and Dentistry, Nagasaki, Japan.
|
|
pubmed:publicationType |
Journal Article
|
