Source:http://linkedlifedata.com/resource/pubmed/id/15648064
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
3
|
| pubmed:dateCreated |
2005-1-18
|
| pubmed:abstractText |
A new human coronavirus, HCoV-NL63, was associated recently with bronchiolitis. The current study aimed to examine retrospectively stored specimens for the presence of HCoV-NL63 using nested RT-PCR assays targeting the 1a and 1b genes. The study population was composed of patients with acute respiratory disease warranting presentation to Queensland hospitals. HCoV-NL63 was detected in the nasopharyngeal aspirates (NPA) of 16 of 840 specimens representing 766 patients (2%). HCoV-NL63 positive individuals were diagnosed most commonly with lower respiratory tract (LRT) disease (81%). The clinical diagnosis was commonly supported by an abnormal chest X-ray (56%) together with respiratory distress (50%), wheeze (44%), and rales (25%) on first presentation with HCoV-NL63 infection. All patients positive for HCoV-NL63 required admission to hospital. Among 38% of HCoV-NL63 positive specimens a second pathogen was detected. Sequencing of amplicon from gene 1b revealed more than 99% nucleotide homology with the viral type strains while sequencing amplicon from gene 1a permitted the grouping of viral strains. It was shown that HCoV-NL63 is associated with severe LRT disease in an Australian hospital setting during the cooler months of the year. We propose that HCoV-NL63 is a global and seasonal pathogen of both children and adults associated with severe LRT illness.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
Mar
|
| pubmed:issn |
0146-6615
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| pubmed:author | |
| pubmed:copyrightInfo |
2005 Wiley-Liss, Inc.
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| pubmed:issnType |
Print
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| pubmed:volume |
75
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| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
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| pubmed:pagination |
455-62
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| pubmed:dateRevised |
2006-11-15
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| pubmed:meshHeading |
pubmed-meshheading:15648064-Adolescent,
pubmed-meshheading:15648064-Adult,
pubmed-meshheading:15648064-Aged,
pubmed-meshheading:15648064-Australia,
pubmed-meshheading:15648064-Bronchiolitis,
pubmed-meshheading:15648064-Child,
pubmed-meshheading:15648064-Child, Preschool,
pubmed-meshheading:15648064-Coronavirus,
pubmed-meshheading:15648064-Coronavirus Infections,
pubmed-meshheading:15648064-Female,
pubmed-meshheading:15648064-Humans,
pubmed-meshheading:15648064-Infant,
pubmed-meshheading:15648064-Infant, Newborn,
pubmed-meshheading:15648064-Male,
pubmed-meshheading:15648064-Middle Aged,
pubmed-meshheading:15648064-Nasopharynx,
pubmed-meshheading:15648064-Phylogeny,
pubmed-meshheading:15648064-RNA, Viral,
pubmed-meshheading:15648064-Retrospective Studies,
pubmed-meshheading:15648064-Reverse Transcriptase Polymerase Chain Reaction,
pubmed-meshheading:15648064-Sequence Analysis, DNA,
pubmed-meshheading:15648064-Viral Proteins
|
| pubmed:year |
2005
|
| pubmed:articleTitle |
New human coronavirus, HCoV-NL63, associated with severe lower respiratory tract disease in Australia.
|
| pubmed:affiliation |
Clinical Virology and Molecular Microbiology Research Units, Sir Albert Sakzewski Virus Research Centre, Royal Children's Hospital, Queensland, Australia.
|
| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|