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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
2
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pubmed:dateCreated |
2005-1-12
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pubmed:abstractText |
Resistance to apoptosis has been implicated in the poor response of cancer cells to various anti-tumor agents. Caspase-8 is a family member of executioner caspases associated with tumor necrosis factor (TNF) family death receptors-mediated apoptotic signaling cascade. In this study, to specify caspase-8-mediated apoptotic activity, we examined the anti-tumor effect of adenoviral vector expressing caspase-8 (Adv-caspase-8) in combination with TNF-related apoptosis-inducing ligand (TRAIL) which induces specifically caspase-8 activation. First, we demonstrated that expression procaspase-8 is related to apoptosis sensitivity to TRAIL in pancreatic and colonic cancer cells. In human pancreatic cancer cell line Panc1 which demonstrates low expression of procaspase-8, Adv-caspase-8 transfection strongly augmented TRAIL-induced apoptosis. Adv-caspase-8 similarly enhanced the susceptibility of human colonic cancer cell line Colo320DM to TRAIL. These results suggest that Adv-caspase-8 may be a good combination partner of TRAIL and enables TRAIL to be a more potent anticancer agent in a wide range of adenocarcinoma cells which demonstrate low expression of caspase-8.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Apoptosis Regulatory Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/CASP3 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/CASP8 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Caspase 3,
http://linkedlifedata.com/resource/pubmed/chemical/Caspase 8,
http://linkedlifedata.com/resource/pubmed/chemical/Caspases,
http://linkedlifedata.com/resource/pubmed/chemical/Membrane Glycoproteins,
http://linkedlifedata.com/resource/pubmed/chemical/TNF-Related Apoptosis-Inducing...,
http://linkedlifedata.com/resource/pubmed/chemical/TNFSF10 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Tumor Necrosis Factor-alpha,
http://linkedlifedata.com/resource/pubmed/chemical/Tumor Suppressor Protein p53
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pubmed:status |
MEDLINE
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pubmed:month |
Feb
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pubmed:issn |
1019-6439
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
26
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
537-44
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:15645141-Adenocarcinoma,
pubmed-meshheading:15645141-Adenoviridae,
pubmed-meshheading:15645141-Apoptosis,
pubmed-meshheading:15645141-Apoptosis Regulatory Proteins,
pubmed-meshheading:15645141-Blotting, Western,
pubmed-meshheading:15645141-Carcinoma,
pubmed-meshheading:15645141-Caspase 3,
pubmed-meshheading:15645141-Caspase 8,
pubmed-meshheading:15645141-Caspases,
pubmed-meshheading:15645141-Cell Line, Tumor,
pubmed-meshheading:15645141-Colonic Neoplasms,
pubmed-meshheading:15645141-Dose-Response Relationship, Drug,
pubmed-meshheading:15645141-Gene Transfer Techniques,
pubmed-meshheading:15645141-Genetic Vectors,
pubmed-meshheading:15645141-Humans,
pubmed-meshheading:15645141-Membrane Glycoproteins,
pubmed-meshheading:15645141-Signal Transduction,
pubmed-meshheading:15645141-TNF-Related Apoptosis-Inducing Ligand,
pubmed-meshheading:15645141-Transfection,
pubmed-meshheading:15645141-Tumor Necrosis Factor-alpha,
pubmed-meshheading:15645141-Tumor Suppressor Protein p53
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pubmed:year |
2005
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pubmed:articleTitle |
Adenovirus-mediated gene transfer of caspase-8 sensitizes human adenocarcinoma cells to tumor necrosis factor-related apoptosis-inducing ligand.
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pubmed:affiliation |
Jerome Lipper Multiple Myeloma Center, Dana-Farber Cancer Institute, Boston, MA 02115, USA. hiroshi_yasui@dfci.harvard.edu
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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