Linked Life Data

15645132

Source:http://linkedlifedata.com/resource/pubmed/id/15645132

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
2005-1-12
pubmed:abstractText
We investigated hepatitis B virus (HBV) DNA integration and expression of several proteins involved in the cell cycle and apoptosis, including cyclin A, retinoblastoma protein (pRB), Fas-associated death domain protein (FADD), tumor necrosis factor receptor-associated death domain protein (TRADD), and nuclear factor kappaB (NF-kappaB) in HBV-associated hepatocellular carcinoma (HCC) and liver cirrhosis (LC). Archival HCC and LC specimens were obtained from 35 patients each with HBV infection; 5 normal liver specimens used as controls were also obtained. Polymerase chain reaction and Southern blot hybridization were used to detect the integration of HBV DNA in the HCC and LC specimens. The protein levels were determined by Western blot assay. The difference in HBV DNA integration between HCC and LC and correlation between HBV-encoded X protein (Hbx) integration and protein expression were analyzed statistically. HBV DNA was detected in 33 (94%) of the HCC and LC specimens. HBx integration differed in the HCC [24 (69%)] and LC [14 (40%)] specimens (p=0.015). Sixty percent of the HCC specimens and 6% of the LC specimens had increased cyclin A expression. Also, 34, 37, 69, and 77% of the HCC specimens were positive for pRB, FADD, TRADD, and NF-kappaB expression, whereas 80, 60, 100, and 100% of the LC specimens were positive for pRB, FADD, TRADD, and NF-kappaB expression. Significant correlations between HBx integration and the level of expression of cyclin A (r=0.452; p=0.006), pRB (r=-0.419; p=0.012), and TRADD (r=0.470; p=0.004) were discovered. Therefore, integration of HBV DNA occurred frequently in HCC and LC cases with chronic HBV infection, whereas HBx integration occurred more often in HCC than in LC cases (p=0.015). HBx integration and altered expression of genes is a key to apoptosis and may play important roles in HBV-induced hepatocarcinogenesis.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Feb
pubmed:issn
1019-6439
pubmed:author
pubmed:issnType
Print
pubmed:volume
26
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
467-73
pubmed:dateRevised
2009-11-19
pubmed:meshHeading
pubmed-meshheading:15645132-Apoptosis, pubmed-meshheading:15645132-Arabidopsis Proteins, pubmed-meshheading:15645132-Biopsy, pubmed-meshheading:15645132-Blotting, Southern, pubmed-meshheading:15645132-Blotting, Western, pubmed-meshheading:15645132-Carcinoma, Hepatocellular, pubmed-meshheading:15645132-Cell Transformation, Neoplastic, pubmed-meshheading:15645132-Cyclin A, pubmed-meshheading:15645132-DNA, pubmed-meshheading:15645132-DNA, Viral, pubmed-meshheading:15645132-Fatty Acid Desaturases, pubmed-meshheading:15645132-Fibrosis, pubmed-meshheading:15645132-Gene Expression Regulation, Neoplastic, pubmed-meshheading:15645132-Gene Expression Regulation, Viral, pubmed-meshheading:15645132-Hepatitis B virus, pubmed-meshheading:15645132-Humans, pubmed-meshheading:15645132-Liver Cirrhosis, pubmed-meshheading:15645132-Liver Neoplasms, pubmed-meshheading:15645132-NF-kappa B, pubmed-meshheading:15645132-Protein Structure, Tertiary, pubmed-meshheading:15645132-Retinoblastoma Protein, pubmed-meshheading:15645132-Trans-Activators
pubmed:year
2005
pubmed:articleTitle
Integration of the hepatitis B virus X fragment in hepatocellular carcinoma and its effects on the expression of multiple molecules: a key to the cell cycle and apoptosis.
pubmed:affiliation
Department of General Surgery, Shanghai Jiaotong University Affiliated Shanghai First People's Hospital, Shanghai 200080, P.R. China.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't