Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
2005-1-12
pubmed:abstractText
The aim of the present study was to investigate the involvement of adenosine 3',5'-cyclic monophosphate (cAMP) cascade in the acute impairment of contraction by 17beta-estradiol in porcine coronary arteries, and to elucidate the signaling pathway leading to the activation of this cascade by the hormone. Isometric tension was recorded in isolated rings of porcine coronary arteries. The contraction to U46619 was reduced significantly following 30 min incubation with 1 nM 17beta-estradiol or 1 nM isoproterenol. There was no additive effect when 17beta-estradiol and isoproterenol were administered together. The effect of 17beta-estradiol was mimicked by both the cyclic AMP analogue 8-Br-cAMP and the guanosine 3',5'-cyclic monophosphate (cyclic GMP) analogue 8-Br-cGMP. In rings with and without endothelium, the modulatory effect of 17beta-estradiol was abolished by the adenylyl cyclase inhibitor, SQ 22536, but was unaffected by the guanylyl cyclase inhibitor, ODQ. Both the cAMP antagonist Rp-8-Br-cAMPS and the cGMP antagonist inhibitor Rp-8-Br-cGMPS inhibited the effect of 17beta-estradiol. The effect of 17beta-estradiol was unaffected by the protein kinase A inhibitor, KT5720, but was abolished by the protein kinase G (PKG) inhibitor, KT5823, which also abolished the effect of isoproterenol. These data support our earlier findings that 17beta-estradiol (1 nM) acutely impairs contractile responses of porcine coronary arteries in vitro. This acute effect of 17beta-estradiol involves cAMP in vascular smooth muscles and the activation of PKG.
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/15644870-10051132, http://linkedlifedata.com/resource/pubmed/commentcorrection/15644870-10086974, http://linkedlifedata.com/resource/pubmed/commentcorrection/15644870-10334810, http://linkedlifedata.com/resource/pubmed/commentcorrection/15644870-10435014, http://linkedlifedata.com/resource/pubmed/commentcorrection/15644870-10468219, http://linkedlifedata.com/resource/pubmed/commentcorrection/15644870-10547076, http://linkedlifedata.com/resource/pubmed/commentcorrection/15644870-10581311, http://linkedlifedata.com/resource/pubmed/commentcorrection/15644870-10742293, http://linkedlifedata.com/resource/pubmed/commentcorrection/15644870-10747354, http://linkedlifedata.com/resource/pubmed/commentcorrection/15644870-10785513, http://linkedlifedata.com/resource/pubmed/commentcorrection/15644870-10797124, http://linkedlifedata.com/resource/pubmed/commentcorrection/15644870-11029403, http://linkedlifedata.com/resource/pubmed/commentcorrection/15644870-11134246, http://linkedlifedata.com/resource/pubmed/commentcorrection/15644870-11498504, http://linkedlifedata.com/resource/pubmed/commentcorrection/15644870-11504785, http://linkedlifedata.com/resource/pubmed/commentcorrection/15644870-12411396, http://linkedlifedata.com/resource/pubmed/commentcorrection/15644870-12515753, http://linkedlifedata.com/resource/pubmed/commentcorrection/15644870-12581882, http://linkedlifedata.com/resource/pubmed/commentcorrection/15644870-1311330, http://linkedlifedata.com/resource/pubmed/commentcorrection/15644870-1315649, http://linkedlifedata.com/resource/pubmed/commentcorrection/15644870-14573532, http://linkedlifedata.com/resource/pubmed/commentcorrection/15644870-1870648, http://linkedlifedata.com/resource/pubmed/commentcorrection/15644870-2331772, http://linkedlifedata.com/resource/pubmed/commentcorrection/15644870-3121846, http://linkedlifedata.com/resource/pubmed/commentcorrection/15644870-3568321, http://linkedlifedata.com/resource/pubmed/commentcorrection/15644870-7074225, http://linkedlifedata.com/resource/pubmed/commentcorrection/15644870-7515189, http://linkedlifedata.com/resource/pubmed/commentcorrection/15644870-8041534, http://linkedlifedata.com/resource/pubmed/commentcorrection/15644870-8044949, http://linkedlifedata.com/resource/pubmed/commentcorrection/15644870-8181150, http://linkedlifedata.com/resource/pubmed/commentcorrection/15644870-8287400, http://linkedlifedata.com/resource/pubmed/commentcorrection/15644870-8532268, http://linkedlifedata.com/resource/pubmed/commentcorrection/15644870-8632333, http://linkedlifedata.com/resource/pubmed/commentcorrection/15644870-8841085, http://linkedlifedata.com/resource/pubmed/commentcorrection/15644870-8994444, http://linkedlifedata.com/resource/pubmed/commentcorrection/15644870-9080377, http://linkedlifedata.com/resource/pubmed/commentcorrection/15644870-9733617, http://linkedlifedata.com/resource/pubmed/commentcorrection/15644870-9876315, http://linkedlifedata.com/resource/pubmed/commentcorrection/15644870-9927501
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
http://linkedlifedata.com/resource/pubmed/chemical/15-Hydroxy-11 alpha,9..., http://linkedlifedata.com/resource/pubmed/chemical/8-Bromo Cyclic Adenosine..., http://linkedlifedata.com/resource/pubmed/chemical/8-bromoadenosine-3',5'-cyclic..., http://linkedlifedata.com/resource/pubmed/chemical/8-bromocyclic GMP, http://linkedlifedata.com/resource/pubmed/chemical/8-bromoguanosino-3',5'-cyclic..., http://linkedlifedata.com/resource/pubmed/chemical/9-(tetrahydro-2-furyl)-adenine, http://linkedlifedata.com/resource/pubmed/chemical/Adenine, http://linkedlifedata.com/resource/pubmed/chemical/Adenylate Cyclase, http://linkedlifedata.com/resource/pubmed/chemical/Carbazoles, http://linkedlifedata.com/resource/pubmed/chemical/Cyclic AMP, http://linkedlifedata.com/resource/pubmed/chemical/Cyclic AMP-Dependent Protein Kinases, http://linkedlifedata.com/resource/pubmed/chemical/Cyclic GMP, http://linkedlifedata.com/resource/pubmed/chemical/Cyclic GMP-Dependent Protein Kinases, http://linkedlifedata.com/resource/pubmed/chemical/Estradiol, http://linkedlifedata.com/resource/pubmed/chemical/Indoles, http://linkedlifedata.com/resource/pubmed/chemical/Isoproterenol, http://linkedlifedata.com/resource/pubmed/chemical/KT 5823, http://linkedlifedata.com/resource/pubmed/chemical/Thionucleotides
pubmed:status
MEDLINE
pubmed:month
Jan
pubmed:issn
0007-1188
pubmed:author
pubmed:issnType
Print
pubmed:volume
144
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
71-9
pubmed:dateRevised
2009-11-19
pubmed:meshHeading
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