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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
6
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pubmed:dateCreated |
2005-1-12
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pubmed:abstractText |
Interleukin (IL)-12 is a key factor in cell-mediated immunity that drives the development of Th1 cells and stimulates T lymphocytes and natural killer cells to produce interferon (INF)-gamma. The IL-12B gene, which encodes the p40 subunit of IL-12, is located at chromosome 5q31-33 and a linkage finding for autoimmune thyroid disease (AITD) on 5q31-33 in a Japanese population has been reported. It is also reported that the A/C polymorphism in the 3' untranslated region (UTR) of the IL-12B gene (1188A/C) is associated with IL12B mRNA expression levels. We attempted to determine whether genetic polymorphisms of the IL-12B gene are associated with AITD. One hundred three patients with Hashimoto's thyroiditis, 90 patients with Graves' disease, and 123 healthy control subjects were recruited. We detected the 1188A/C polymorphism using a PCR-RFLP method and the A/T polymorphism in intron 4 of the IL-12B gene using a cycle sequencing method. These IL-12B gene polymorphisms showed strong linkage disequilibrium, and their genotype and allele frequencies in the patients did not differ from those in the control subjects. Our results suggest that IL-12B gene polymorphisms were unlikely to have an effect on the development of Hashimoto's thyroiditis or Graves' disease in Japanese patients.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/3' Untranslated Regions,
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, CD,
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, Differentiation,
http://linkedlifedata.com/resource/pubmed/chemical/CTLA-4 Antigen,
http://linkedlifedata.com/resource/pubmed/chemical/CTLA4 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/DNA,
http://linkedlifedata.com/resource/pubmed/chemical/IL12B protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Interleukin-12,
http://linkedlifedata.com/resource/pubmed/chemical/Interleukin-12 Subunit p40
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pubmed:status |
MEDLINE
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pubmed:month |
Dec
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pubmed:issn |
0918-8959
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
51
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
609-13
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pubmed:dateRevised |
2011-11-17
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pubmed:meshHeading |
pubmed-meshheading:15644581-3' Untranslated Regions,
pubmed-meshheading:15644581-Antigens, CD,
pubmed-meshheading:15644581-Antigens, Differentiation,
pubmed-meshheading:15644581-CTLA-4 Antigen,
pubmed-meshheading:15644581-DNA,
pubmed-meshheading:15644581-Female,
pubmed-meshheading:15644581-Genotype,
pubmed-meshheading:15644581-Graves Disease,
pubmed-meshheading:15644581-Humans,
pubmed-meshheading:15644581-Interleukin-12,
pubmed-meshheading:15644581-Interleukin-12 Subunit p40,
pubmed-meshheading:15644581-Japan,
pubmed-meshheading:15644581-Linkage Disequilibrium,
pubmed-meshheading:15644581-Male,
pubmed-meshheading:15644581-Middle Aged,
pubmed-meshheading:15644581-Polymerase Chain Reaction,
pubmed-meshheading:15644581-Polymorphism, Single Nucleotide,
pubmed-meshheading:15644581-Sequence Analysis, DNA,
pubmed-meshheading:15644581-Thyroiditis, Autoimmune
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pubmed:year |
2004
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pubmed:articleTitle |
Lack of association between IL-12B gene polymorphism and autoimmune thyroid disease in Japanese patients.
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pubmed:affiliation |
Department of Endocrinology, Metabolism and Nephrology, Kochi Medical School, Kochi University, Nankoku, Kochi 783-8505, Japan.
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pubmed:publicationType |
Journal Article
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