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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
2
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pubmed:dateCreated |
2005-1-11
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pubmed:abstractText |
The results presented demonstrate the expression of pfkfb4 gene in adult testis and in a mouse spermatogonia germ cell line (GC-1spg). The genomic organization of the human pfkfb4 gene shows the existence of 14 exons and 13 introns, spanning 45 kb. A detailed analysis of the 5'-flanking region by transient transfection assays with different 5'-deletion promoter constructs in GC-1spg and mouse sertoli cells (TM-4), allows us to define the minimal promoter unit, containing several GC-rich and ETF sequences along the first -141 nucleotides involved in basal expression. This gene is activated by serum and chemical hypoxia (CoCl(2) treatment) whereas beta-estradiol decreases its expression.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Jan
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pubmed:issn |
0014-5793
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
17
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pubmed:volume |
579
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
357-62
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pubmed:dateRevised |
2008-11-21
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pubmed:meshHeading |
pubmed-meshheading:15642344-5' Flanking Region,
pubmed-meshheading:15642344-Animals,
pubmed-meshheading:15642344-Base Sequence,
pubmed-meshheading:15642344-Cell Line,
pubmed-meshheading:15642344-Exons,
pubmed-meshheading:15642344-Gene Expression,
pubmed-meshheading:15642344-Gene Expression Regulation,
pubmed-meshheading:15642344-Genes, Reporter,
pubmed-meshheading:15642344-Germ Cells,
pubmed-meshheading:15642344-Humans,
pubmed-meshheading:15642344-Introns,
pubmed-meshheading:15642344-Luciferases,
pubmed-meshheading:15642344-Male,
pubmed-meshheading:15642344-Mice,
pubmed-meshheading:15642344-Molecular Sequence Data,
pubmed-meshheading:15642344-Phosphofructokinase-2,
pubmed-meshheading:15642344-Promoter Regions, Genetic,
pubmed-meshheading:15642344-RNA, Messenger,
pubmed-meshheading:15642344-Rats,
pubmed-meshheading:15642344-Sequence Deletion,
pubmed-meshheading:15642344-Spermatogonia,
pubmed-meshheading:15642344-Testis,
pubmed-meshheading:15642344-Tissue Distribution
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pubmed:year |
2005
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pubmed:articleTitle |
Specific expression of pfkfb4 gene in spermatogonia germ cells and analysis of its 5'-flanking region.
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pubmed:affiliation |
Unitat de Bioquímica i Biologia Molecular, Departament de Ciències Fisiològiques II, Campus de Bellvitge, Universitat de Barcelona, Feixa Llarga s/n E-08907 L'Hospitalet, Spain.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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