Linked Life Data

15639917

Source:http://linkedlifedata.com/resource/pubmed/id/15639917

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
5
pubmed:dateCreated
2005-1-10
pubmed:abstractText
To clone goat beta-lactoglobulin (BLG) gene,two fragments were amplified from goat genomic DNA by LD-PCR method. The fragments were inserted in T-vectors before being spliced into the whole 7.2 kb BLG gene at a single restriction enzyme site of NarI. Consequently, the eukaryotic expression vector was constructed. All the clones were proved to be correct by restriction enzyme cutting and sequencing analysis. Six Founders (3 male symbol, 3 female symbol) of goat BLG transgenic mice were obtained by microinjection and BLG genes integration were confirmed by both PCR and Southern blot analyses. The milk was collected from two lactating female transgenic mice and goat BLG protein contents were measured with ELISA. The results showed that goat BLG protein in milk of the two mice were 23.49 mg/mL and 2.19 mg/mL, respectively.
pubmed:language
chi
pubmed:journal
pubmed:status
PubMed-not-MEDLINE
pubmed:month
Sep
pubmed:issn
0253-9772
pubmed:author
pubmed:issnType
Print
pubmed:volume
25
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
499-503
pubmed:dateRevised
2006-11-15
pubmed:year
2003
pubmed:articleTitle
[Goat beta-lactoglobulin gene cloning and high expression in the mammary gland of transgenic mice].
pubmed:affiliation
Institute of Medical Genetics, Shanghai Jiaotong University, Shanghai 200040, China.
pubmed:publicationType
Journal Article, English Abstract