Linked Life Data

15639839

Source:http://linkedlifedata.com/resource/pubmed/id/15639839

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
pubmed:issue
2
pubmed:dateCreated
2005-1-10
pubmed:abstractText
A synthesized double-stranded oligomeric nucleotide encoding 11-amino acid TAT protein transduction domain3 was inserted into pET28a vector after 6 histidine coding sequence, LacZ gene from pcDNA4/Myc-His/LacZ was digested by EcoR I and Hind III, then cloned into pET28a-TAT. The highly expressed TAT-beta-galactosidase was purified by affinity chromatography. TAT-beta-Gal fusion protein can across vascular smooth muscle cells in vitro. This result open new possibility for direct delivery of protein into tissues for therapy.
pubmed:language
chi
pubmed:journal
pubmed:status
PubMed-not-MEDLINE
pubmed:month
Mar
pubmed:issn
0253-9772
pubmed:author
pubmed:issnType
Print
pubmed:volume
25
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
141-4
pubmed:dateRevised
2006-11-15
pubmed:year
2003
pubmed:articleTitle
[Constructing the recombinant of pET28a-TAT-LacZ].
pubmed:affiliation
Department of Clinical laboratory, Shiyan Peoples Hospital of Yunyang medical college, Shiyan, Hubei 442000, China. graceyan@netease.com
pubmed:publicationType
Journal Article, English Abstract