Linked Life Data

1563981

Source:http://linkedlifedata.com/resource/pubmed/id/1563981

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
1992-5-15
pubmed:abstractText
Recipients enrolled in the Collaborative Corneal Transplantation Studies were retyped as part of a quality assurance program. Because the observed percentage of HLA-DR homozygosity on original typing was more than twice as high as expected from CCTS allele frequencies, the sample selected for retyping was heavily weighted with patients whose original typing identified fewer than two DR antigens. Retyping was performed in a different laboratory from the laboratory performing the original typing. For the 129 patients who were retyped, agreement between the original and retyping laboratories was 88% for HLA-A, 79% for HLA-B, and 55% for HLA-DR. When criteria was relaxed to consider only discrepancies involving readily identifiable antigens, the agreement improved to 95% for HLA-A, 91% for HLA-B, and 59% for HLA-DR. Identification of a second HLA-DR antigen on retyping when only one DR antigen had been identified on original typing was by far the most common form of disagreement. There were no significant differences in the amount of disagreement among the laboratories. Of special interest is that 50% of the discrepancies involved DR3, DR5, and/or DR6, which have structural similarities. Based on the results of the project, we recommend: (1) replicate testing for all DR typing; and (2) retyping using a second source of antiserum for all subjects having DR blanks.
pubmed:grant
pubmed:commentsCorrections
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Feb
pubmed:issn
0198-8859
pubmed:author
pubmed:issnType
Print
pubmed:volume
33
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
122-8
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed:year
1992
pubmed:articleTitle
Reproducibility of HLA-A, B, and DR typing using peripheral blood samples: results of retyping in the collaborative corneal transplantation studies. Collaborative Corneal Transplantation Studies Group (corrected)
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S.