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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
2
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pubmed:dateCreated |
2005-1-6
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pubmed:abstractText |
The mechanisms regulating T lymphocyte migration within the extracellular matrix are not understood. We show in this study that the thrombospondin-1 binding site of calreticulin, spanning aa 19-32, is a major triggering factor for T cell motility and migration within a three-dimensional collagen type 1 matrix, and that exogenous motogenic factors such as chemokines can stimulate migration via a calreticulin-thrombospondin-1 pathway. Endogenous calreticulin binding to the N-terminal domain of endogenous thrombospondin-1 elicited a motogenic signal to the T cells through the C-terminal domain of thrombospondin-1 and its cell surface receptor integrin-associated protein (CD47). Our data further revealed that thrombospondin-1 was expressed on the cell surface with a high turnover, and that PI3K and the Janus family of tyrosine kinases were required for T cell motility mediated through calreticulin, thrombospondin-1, and CD47. These results unveil an autocrine mechanism of calreticulin-thrombospondin-1-CD47 interaction for the control of T cell motility and migration within three-dimensional extracellular matrix substrata.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
AIM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Adjuvants, Immunologic,
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, CD,
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, CD47,
http://linkedlifedata.com/resource/pubmed/chemical/CD47 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/CXCL12 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Calreticulin,
http://linkedlifedata.com/resource/pubmed/chemical/Chemokine CXCL12,
http://linkedlifedata.com/resource/pubmed/chemical/Chemokines, CXC,
http://linkedlifedata.com/resource/pubmed/chemical/Collagen Type I,
http://linkedlifedata.com/resource/pubmed/chemical/Heparin,
http://linkedlifedata.com/resource/pubmed/chemical/Peptide Fragments,
http://linkedlifedata.com/resource/pubmed/chemical/Thrombospondin 1,
http://linkedlifedata.com/resource/pubmed/chemical/Zinc
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pubmed:status |
MEDLINE
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pubmed:month |
Jan
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pubmed:issn |
0022-1767
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
15
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pubmed:volume |
174
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
654-61
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pubmed:dateRevised |
2007-11-15
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pubmed:meshHeading |
pubmed-meshheading:15634883-Adjuvants, Immunologic,
pubmed-meshheading:15634883-Amino Acid Sequence,
pubmed-meshheading:15634883-Antigens, CD,
pubmed-meshheading:15634883-Antigens, CD47,
pubmed-meshheading:15634883-Autocrine Communication,
pubmed-meshheading:15634883-Binding Sites,
pubmed-meshheading:15634883-Calreticulin,
pubmed-meshheading:15634883-Cell Membrane,
pubmed-meshheading:15634883-Cell Migration Inhibition,
pubmed-meshheading:15634883-Cell Movement,
pubmed-meshheading:15634883-Cells, Cultured,
pubmed-meshheading:15634883-Chemokine CXCL12,
pubmed-meshheading:15634883-Chemokines, CXC,
pubmed-meshheading:15634883-Collagen Type I,
pubmed-meshheading:15634883-Heparin,
pubmed-meshheading:15634883-Humans,
pubmed-meshheading:15634883-Molecular Sequence Data,
pubmed-meshheading:15634883-Peptide Fragments,
pubmed-meshheading:15634883-Protein Transport,
pubmed-meshheading:15634883-T-Lymphocytes,
pubmed-meshheading:15634883-Thrombospondin 1,
pubmed-meshheading:15634883-Zinc
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pubmed:year |
2005
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pubmed:articleTitle |
Autocrine regulation of T cell motility by calreticulin-thrombospondin-1 interaction.
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pubmed:affiliation |
Division of Clinical Immunology, Department of Clinical Microbiology, Umeå University, Umeå, Sweden.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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