Source:http://linkedlifedata.com/resource/pubmed/id/15634879
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
2
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| pubmed:dateCreated |
2005-1-6
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| pubmed:abstractText |
In this study, we suggest that CD8 levels on T cells are not static, but can change and, as a result, modulate CD8(+) T cell responses. We describe three models of CD8 modulation using novel weak-agonist (K1A) and super-agonist (C2A) altered peptide ligands of the HY smcy peptide. First, we used peripheral nonresponsive CD8(low) T cells produced after peripheral HY-D(b) MHC class I tetramer stimulation of female HY TCR transgenic and wild-type mice. Second, we used genetically lowered CD8(int) T cells from heterozygote CD8(+/0) mice. Finally, we used pre-existing nonresponsive CD8(low) T cells from male HY TCR transgenic mice. In CD8(low) and CD8(high) mice, presence of a lower level of CD8 greatly decreased the avidity of the peptide-MHC for HY TCR as reflected by avidity (K(D)) and dissociation constant (T(1/2)) measurements. All three models demonstrated that lowering CD8 levels resulted in the requirement for a higher avidity peptide-MHC interaction with the TCR to respond equivalently to unmanipulated CD8(high) T cells of the same specificity. Additionally, direct injections of wild-type HY-D(b) and C2A-D(b) tetramers into female HY TCR or female B6 mice induced a high frequency of peripheral nonresponsive CD8(low) T cells, yet C2A-D(b) was superior in inducing a primed CD8(+)CD44(+) memory population. The ability to dynamically modulate the size and responsiveness of an Ag-specific T cell pool by "CD8 tuning" of the T cell during the early phases of an immune response has important implications for the balance of responsiveness, memory, and tolerance.
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| pubmed:grant | |
| pubmed:language |
eng
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| pubmed:journal | |
| pubmed:citationSubset |
AIM
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| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, CD8,
http://linkedlifedata.com/resource/pubmed/chemical/Epitopes, T-Lymphocyte,
http://linkedlifedata.com/resource/pubmed/chemical/H-Y Antigen,
http://linkedlifedata.com/resource/pubmed/chemical/Ligands,
http://linkedlifedata.com/resource/pubmed/chemical/Peptides,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Antigen, T-Cell
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| pubmed:status |
MEDLINE
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| pubmed:month |
Jan
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| pubmed:issn |
0022-1767
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| pubmed:author | |
| pubmed:issnType |
Print
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| pubmed:day |
15
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| pubmed:volume |
174
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| pubmed:owner |
NLM
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| pubmed:authorsComplete |
Y
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| pubmed:pagination |
619-27
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| pubmed:dateRevised |
2007-11-14
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| pubmed:meshHeading |
pubmed-meshheading:15634879-Animals,
pubmed-meshheading:15634879-Antigens, CD8,
pubmed-meshheading:15634879-CD8-Positive T-Lymphocytes,
pubmed-meshheading:15634879-Cell Differentiation,
pubmed-meshheading:15634879-Cytotoxicity, Immunologic,
pubmed-meshheading:15634879-Down-Regulation,
pubmed-meshheading:15634879-Epitopes, T-Lymphocyte,
pubmed-meshheading:15634879-Female,
pubmed-meshheading:15634879-H-Y Antigen,
pubmed-meshheading:15634879-Ligands,
pubmed-meshheading:15634879-Lymphocyte Activation,
pubmed-meshheading:15634879-Male,
pubmed-meshheading:15634879-Mice,
pubmed-meshheading:15634879-Mice, Inbred C57BL,
pubmed-meshheading:15634879-Mice, Transgenic,
pubmed-meshheading:15634879-Peptides,
pubmed-meshheading:15634879-Protein Binding,
pubmed-meshheading:15634879-Receptors, Antigen, T-Cell,
pubmed-meshheading:15634879-Sex Characteristics,
pubmed-meshheading:15634879-T-Lymphocyte Subsets,
pubmed-meshheading:15634879-Transplantation Tolerance
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| pubmed:year |
2005
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| pubmed:articleTitle |
Peripheral "CD8 tuning" dynamically modulates the size and responsiveness of an antigen-specific T cell pool in vivo.
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| pubmed:affiliation |
Department of Microbiology and Immunology, University of North Carolina, Chapel Hill 27599, USA. robmaile@bellsouth.net
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| pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
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