Linked Life Data

15634762

Source:http://linkedlifedata.com/resource/pubmed/id/15634762

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
2005-1-6
pubmed:abstractText
Ependymal cells on the walls of brain ventricles play essential roles in the transport of CSF and in brain homeostasis. It has been suggested that ependymal cells also function as stem cells. However, the proliferative capacity of mature ependymal cells remains controversial, and the developmental origin of these cells is not known. Using confocal or electron microscopy (EM) of adult mice that received bromodeoxyuridine (BrdU) or [3H]thymidine for several weeks, we found no evidence that ependymal cells proliferate. In contrast, ependymal cells were labeled by BrdU administration during embryonic development. The majority of them are born between embryonic day 14 (E14) and E16. Interestingly, we found that the maturation of ependymal cells and the formation of cilia occur significantly later, during the first postnatal week. We analyzed the early postnatal ventricular zone at the EM and found a subpopulation of radial glia in various stages of transformation into ependymal cells. These cells often had deuterosomes. To directly test whether radial glia give rise to ependymal cells, we used a Cre-lox recombination strategy to genetically tag radial glia in the neonatal brain and follow their progeny. We found that some radial glia in the lateral ventricular wall transform to give rise to mature ependymal cells. This work identifies the time of birth and early stages in the maturation of ependymal cells and demonstrates that these cells are derived from radial glia. Our results indicate that ependymal cells are born in the embryonic and early postnatal brain and that they do not divide after differentiation. The postmitotic nature of ependymal cells strongly suggests that these cells do not function as neural stem cells in the adult.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jan
pubmed:issn
1529-2401
pubmed:author
pubmed:issnType
Electronic
pubmed:day
5
pubmed:volume
25
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
10-8
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed-meshheading:15634762-Age Factors, pubmed-meshheading:15634762-Amino Acid Transport System X-AG, pubmed-meshheading:15634762-Animals, pubmed-meshheading:15634762-Bromodeoxyuridine, pubmed-meshheading:15634762-Cell Differentiation, pubmed-meshheading:15634762-Cell Lineage, pubmed-meshheading:15634762-Cell Nucleus, pubmed-meshheading:15634762-Cilia, pubmed-meshheading:15634762-Ependyma, pubmed-meshheading:15634762-Glutamate Plasma Membrane Transport Proteins, pubmed-meshheading:15634762-Immunohistochemistry, pubmed-meshheading:15634762-Male, pubmed-meshheading:15634762-Mice, pubmed-meshheading:15634762-Mitosis, pubmed-meshheading:15634762-Neuroglia, pubmed-meshheading:15634762-Organelles, pubmed-meshheading:15634762-S100 Proteins, pubmed-meshheading:15634762-Stem Cells, pubmed-meshheading:15634762-Symporters, pubmed-meshheading:15634762-Thymidine, pubmed-meshheading:15634762-Time Factors, pubmed-meshheading:15634762-Tritium
pubmed:year
2005
pubmed:articleTitle
Adult ependymal cells are postmitotic and are derived from radial glial cells during embryogenesis.
pubmed:affiliation
Department of Neurological Surgery and Program in Developmental and Stem Cell Biology, University of California San Francisco, San Francisco, California 94143, USA.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't, Research Support, N.I.H., Extramural