rdf:type |
|
lifeskim:mentions |
|
pubmed:issue |
1
|
pubmed:dateCreated |
2005-1-6
|
pubmed:abstractText |
In eukaryotes, CpG methylation is an epigenetic DNA modification that is important for heterochromatin formation. Centromere protein B (CENP-B) specifically binds to the centromeric 17 base-pair CENP-B box DNA, which contains two CpG dinucleotides. In this study, we tested complex formation by the DNA-binding domain of CENP-B with methylated and unmethylated CENP-B box DNAs, and found that CENP-B preferentially binds to the unmethylated CENP-B box DNA. Competition analyses revealed that the affinity of CENP-B for the CENP-B box DNA is reduced nearly to the level of nonspecific DNA binding by CpG methylation.
|
pubmed:language |
eng
|
pubmed:journal |
|
pubmed:citationSubset |
IM
|
pubmed:chemical |
|
pubmed:status |
MEDLINE
|
pubmed:month |
Jan
|
pubmed:issn |
1742-464X
|
pubmed:author |
|
pubmed:issnType |
Print
|
pubmed:volume |
272
|
pubmed:owner |
NLM
|
pubmed:authorsComplete |
Y
|
pubmed:pagination |
282-9
|
pubmed:dateRevised |
2006-11-15
|
pubmed:meshHeading |
pubmed-meshheading:15634350-Autoantigens,
pubmed-meshheading:15634350-Base Sequence,
pubmed-meshheading:15634350-Centromere Protein B,
pubmed-meshheading:15634350-Chromosomal Proteins, Non-Histone,
pubmed-meshheading:15634350-CpG Islands,
pubmed-meshheading:15634350-DNA,
pubmed-meshheading:15634350-DNA-Binding Proteins,
pubmed-meshheading:15634350-Humans,
pubmed-meshheading:15634350-Models, Molecular,
pubmed-meshheading:15634350-Protein Binding
|
pubmed:year |
2005
|
pubmed:articleTitle |
CpG methylation of the CENP-B box reduces human CENP-B binding.
|
pubmed:affiliation |
Protein Research Group, RIKEN Genomic Sciences Center, Suehiro-cho, Tsurumi, Yokohama, Japan.
|
pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|