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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
1
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pubmed:dateCreated |
2005-1-6
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pubmed:abstractText |
In eukaryotes, CpG methylation is an epigenetic DNA modification that is important for heterochromatin formation. Centromere protein B (CENP-B) specifically binds to the centromeric 17 base-pair CENP-B box DNA, which contains two CpG dinucleotides. In this study, we tested complex formation by the DNA-binding domain of CENP-B with methylated and unmethylated CENP-B box DNAs, and found that CENP-B preferentially binds to the unmethylated CENP-B box DNA. Competition analyses revealed that the affinity of CENP-B for the CENP-B box DNA is reduced nearly to the level of nonspecific DNA binding by CpG methylation.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Jan
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pubmed:issn |
1742-464X
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
272
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
282-9
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:15634350-Autoantigens,
pubmed-meshheading:15634350-Base Sequence,
pubmed-meshheading:15634350-Centromere Protein B,
pubmed-meshheading:15634350-Chromosomal Proteins, Non-Histone,
pubmed-meshheading:15634350-CpG Islands,
pubmed-meshheading:15634350-DNA,
pubmed-meshheading:15634350-DNA-Binding Proteins,
pubmed-meshheading:15634350-Humans,
pubmed-meshheading:15634350-Models, Molecular,
pubmed-meshheading:15634350-Protein Binding
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pubmed:year |
2005
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pubmed:articleTitle |
CpG methylation of the CENP-B box reduces human CENP-B binding.
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pubmed:affiliation |
Protein Research Group, RIKEN Genomic Sciences Center, Suehiro-cho, Tsurumi, Yokohama, Japan.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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