Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
4
pubmed:dateCreated
1992-5-15
pubmed:abstractText
High resolution digital video imaging has been employed to monitor the spatial and temporal development of agonist-induced cytosolic Ca2+ signals in fura 2-loaded exocrine acinar cells. Enzymatically isolated mouse pancreatic and lacrimal acinar cells or small acinar cell clusters were used. These retain their morphological polarity so that the secretory granules in individual cells are located at one pole, the secretory pole. In acinar cell clusters the granules are located centrally, oriented to surround what would be in situ referred to as the lumen. In pancreatic and lacrimal acinar cells inositol-1,4,5-triphosphate-generating agonists [acetylcholine (ACh) and cholecystokinin octapeptide (CCK) for the pancreas and ACh in the lacrimal gland] give rise to a rapidly spreading Ca2+ signal that is initiated at the secretory pole of the cells. The initial increase in [Ca2+]i in the luminal pole is independent of extracellular Ca2+ indicating that the earliest detectable intracellular Ca2+ release is specifically located at the secretory pole. In lacrimal acinar cells ATP acts as an extracellular agonist, independent of phosphoinositide metabolism to activate a receptor-operated calcium influx pathway which, as for ACh, gives rise firstly to an increase in intracellular Ca2+ concentration in the secretory pole. We propose that this polar rise in intracellular Ca2+ concentration is due to Ca(2+)-induced Ca2+ release. By contrast, when Ca2+ release and Ca2+ influx are induced in the absence of receptor activation by thapsigargin and ionomycin, the Ca2+ signal develops diffusely and slowly with no localization to the secretory pole.(ABSTRACT TRUNCATED AT 250 WORDS)
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/1563357-1096303, http://linkedlifedata.com/resource/pubmed/commentcorrection/1563357-1375629, http://linkedlifedata.com/resource/pubmed/commentcorrection/1563357-1375633, http://linkedlifedata.com/resource/pubmed/commentcorrection/1563357-1662286, http://linkedlifedata.com/resource/pubmed/commentcorrection/1563357-1690123, http://linkedlifedata.com/resource/pubmed/commentcorrection/1563357-1692540, http://linkedlifedata.com/resource/pubmed/commentcorrection/1563357-1701691, http://linkedlifedata.com/resource/pubmed/commentcorrection/1563357-1701852, http://linkedlifedata.com/resource/pubmed/commentcorrection/1563357-1705883, http://linkedlifedata.com/resource/pubmed/commentcorrection/1563357-1830587, http://linkedlifedata.com/resource/pubmed/commentcorrection/1563357-1939077, http://linkedlifedata.com/resource/pubmed/commentcorrection/1563357-1954647, http://linkedlifedata.com/resource/pubmed/commentcorrection/1563357-1959657, http://linkedlifedata.com/resource/pubmed/commentcorrection/1563357-2008014, http://linkedlifedata.com/resource/pubmed/commentcorrection/1563357-2059990, http://linkedlifedata.com/resource/pubmed/commentcorrection/1563357-2105938, http://linkedlifedata.com/resource/pubmed/commentcorrection/1563357-2138778, http://linkedlifedata.com/resource/pubmed/commentcorrection/1563357-2242382, http://linkedlifedata.com/resource/pubmed/commentcorrection/1563357-2293199, http://linkedlifedata.com/resource/pubmed/commentcorrection/1563357-2454074, http://linkedlifedata.com/resource/pubmed/commentcorrection/1563357-2482887, http://linkedlifedata.com/resource/pubmed/commentcorrection/1563357-2492098, http://linkedlifedata.com/resource/pubmed/commentcorrection/1563357-2498663, http://linkedlifedata.com/resource/pubmed/commentcorrection/1563357-2535675, http://linkedlifedata.com/resource/pubmed/commentcorrection/1563357-2536624, http://linkedlifedata.com/resource/pubmed/commentcorrection/1563357-2580043, http://linkedlifedata.com/resource/pubmed/commentcorrection/1563357-2721487, http://linkedlifedata.com/resource/pubmed/commentcorrection/1563357-2768340, http://linkedlifedata.com/resource/pubmed/commentcorrection/1563357-2848507, http://linkedlifedata.com/resource/pubmed/commentcorrection/1563357-3012552, http://linkedlifedata.com/resource/pubmed/commentcorrection/1563357-3127239, http://linkedlifedata.com/resource/pubmed/commentcorrection/1563357-3141216, http://linkedlifedata.com/resource/pubmed/commentcorrection/1563357-3304132, http://linkedlifedata.com/resource/pubmed/commentcorrection/1563357-3755550, http://linkedlifedata.com/resource/pubmed/commentcorrection/1563357-3838314, http://linkedlifedata.com/resource/pubmed/commentcorrection/1563357-3997871, http://linkedlifedata.com/resource/pubmed/commentcorrection/1563357-4373477, http://linkedlifedata.com/resource/pubmed/commentcorrection/1563357-6841449, http://linkedlifedata.com/resource/pubmed/commentcorrection/1563357-701369
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Apr
pubmed:issn
0261-4189
pubmed:author
pubmed:issnType
Print
pubmed:volume
11
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1623-9
pubmed:dateRevised
2010-9-7
pubmed:meshHeading
pubmed:year
1992
pubmed:articleTitle
Spatial and temporal distribution of agonist-evoked cytoplasmic Ca2+ signals in exocrine acinar cells analysed by digital image microscopy.
pubmed:affiliation
MRC Secretory Control Research Group, University of Liverpool, UK.
pubmed:publicationType
Journal Article, In Vitro, Research Support, Non-U.S. Gov't