Source:http://linkedlifedata.com/resource/pubmed/id/15629796
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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
3
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pubmed:dateCreated |
2005-1-4
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pubmed:abstractText |
The possibility of producing quail germline chimeras by the transfer of gonadal primordial germ cells (gPGCs) into recipient embryos was investigated. Japanese quail of the black (D: homozygous for the autosomal incomplete dominant gene D) and wild-type plumage (WP: d+/d+) strains were used as donors and recipients, respectively. Gonadal cells were retrieved from the gonads of 5-day-old D embryos, and gPGCs were enriched by magnetism-activated cell sorting. Fresh (noncultured) gPGCs or those isolated after culture for 3 days with gonadal stromal cells present in the mixed cell population were introduced into the dorsal aorta of 2-day-old recipient WP embryos. Hatchability of the recipient embryos was 23.7% (31/131) and 34.4% (31/90) for those transfused with cultured or noncultured gPGCs, respectively. Of the hatched quail, 28 acquired sexual maturity; among these animals, 7.1% (1/14) and 21.4% (3/14) of those that received cultured or noncultured gPGCs, respectively, were proved to be germline chimeras. The percentage of germline transmission to the donor-derived gametes in the chimeras that received cultured and noncultured gPGCs were 1.9 and 2.2-4.7%, respectively. In conclusion, quail gPGCs retrieved from 5-day-old embryos were thus transmitted in the germline after their transfer to quail embryos of a different strain. This property of the gPGCs was not adversely affected by culture for up to 3 days.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:status |
MEDLINE
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pubmed:month |
Feb
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pubmed:issn |
0093-691X
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:volume |
63
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
774-82
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:15629796-Animals,
pubmed-meshheading:15629796-Aorta,
pubmed-meshheading:15629796-Cells, Cultured,
pubmed-meshheading:15629796-Chimera,
pubmed-meshheading:15629796-Coturnix,
pubmed-meshheading:15629796-Germ Cells,
pubmed-meshheading:15629796-Gonads,
pubmed-meshheading:15629796-Microinjections,
pubmed-meshheading:15629796-Time Factors
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pubmed:year |
2005
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pubmed:articleTitle |
Production of quail (Coturnix japonica) germline chimeras by transfer of gonadal primordial germ cells into recipient embryos.
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pubmed:affiliation |
Avicore Biotechnology Institute, Uiwang City, Kyonggi Province 437-020, South Korea.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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