Source:http://linkedlifedata.com/resource/pubmed/id/15629432
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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
1
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pubmed:dateCreated |
2005-1-4
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pubmed:abstractText |
Somatic hypermutation (SHM) of immunoglobulin variable (V) region genes occurs in the germinal center (GC) B cells during immune responses, depending on activation-induced cytidine deaminase (AID). SHM is associated with resected double-strand DNA breaks (DSBs) which were shown to occur specifically in rearranged V regions in the GC B cells and CD40-stimulated B cells expressing AID. So far, endonucleases responsible for the DSBs have not been identified. Here we show that DNase gamma, a member of DNase I family of endonucleases, is expressed in GC B cells and CD40-stimulated B cells. Overexpression of DNase gamma in the mutation-competent Ramos B-cell line resulted in a marked increase in the resected but not blunt DSBs in the V region. Conversely, a selective DNase gamma inhibitor, DR396, suppressed the generation of the resected DSBs. These results suggest that DNase gamma is involved in the generation of resected DSBs associated with SHM.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical | |
pubmed:status |
MEDLINE
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pubmed:month |
Feb
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pubmed:issn |
0006-291X
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:day |
4
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pubmed:volume |
327
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
76-83
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:15629432-Animals,
pubmed-meshheading:15629432-B-Lymphocytes,
pubmed-meshheading:15629432-Cell Line,
pubmed-meshheading:15629432-DNA,
pubmed-meshheading:15629432-DNA Damage,
pubmed-meshheading:15629432-Endodeoxyribonucleases,
pubmed-meshheading:15629432-Gene Expression,
pubmed-meshheading:15629432-Genes, Immunoglobulin,
pubmed-meshheading:15629432-Humans,
pubmed-meshheading:15629432-Mice,
pubmed-meshheading:15629432-RNA, Messenger,
pubmed-meshheading:15629432-Transfection
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pubmed:year |
2005
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pubmed:articleTitle |
Involvement of DNase gamma in the resected double-strand DNA breaks in immunoglobulin genes.
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pubmed:affiliation |
Division of Molecular Biology, Research Institute for Biological Sciences, Tokyo University of Science, 2669 Yamazaki, Noda, Chiba 278-0022, Japan.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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