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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
2
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pubmed:dateCreated |
2005-1-4
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pubmed:databankReference |
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pubmed:abstractText |
Human ZNF295 protein harbors a BTB/POZ domain and multiple krüppel (C(2)H(2)) type zinc finger domains, and thus belongs to a family of POK (POZ and krüppel) transcription factor. We have identified many transcript variants generated by the alternative splicing in 5' non-coding exons, an intra-exonic splicing in a coding region, and the use of three polyadenylation signals in the 3' UTR. The intra-exonic splicing removes 603-bp coding sequence, and thus ZNF295 gene produces two protein isoforms: ZNF295L with 1066 amino acid residues and ZNF295S with 865 amino acid residues, containing 9 and 5 zinc finger domains, respectively. ZNF295 is ubiquitously expressed in human fetal and adult tissues. Analysis of transcription activity of ZNF295 using various promoter-reporters demonstrated that ZNF295 acts as a transcription repressor, and contains two separate regions for repression activity: the BTB/POZ domain and the central region between BTB/POZ and ZF domains. Both ZNF295L and ZNF295S could interact not only with themselves and each other, but also with another POK protein ZFP161 known to function as a transcription repressor and an activator. We postulated that ZNF295 may be involved in the bi-directional control of gene expression in concert with ZFP161.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Feb
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pubmed:issn |
0006-291X
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
11
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pubmed:volume |
327
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
615-27
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pubmed:dateRevised |
2007-11-15
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pubmed:meshHeading |
pubmed-meshheading:15629158-Amino Acid Sequence,
pubmed-meshheading:15629158-Cell Line,
pubmed-meshheading:15629158-Cloning, Molecular,
pubmed-meshheading:15629158-DNA-Binding Proteins,
pubmed-meshheading:15629158-Exons,
pubmed-meshheading:15629158-Gene Expression Profiling,
pubmed-meshheading:15629158-Humans,
pubmed-meshheading:15629158-Introns,
pubmed-meshheading:15629158-Kruppel-Like Transcription Factors,
pubmed-meshheading:15629158-Molecular Sequence Data,
pubmed-meshheading:15629158-Protein Binding,
pubmed-meshheading:15629158-Protein Structure, Tertiary,
pubmed-meshheading:15629158-Protein Transport,
pubmed-meshheading:15629158-Repressor Proteins,
pubmed-meshheading:15629158-Sequence Alignment,
pubmed-meshheading:15629158-Transcription, Genetic,
pubmed-meshheading:15629158-Transcription Factors,
pubmed-meshheading:15629158-Zinc Fingers
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pubmed:year |
2005
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pubmed:articleTitle |
Novel human BTB/POZ domain-containing zinc finger protein ZNF295 is directly associated with ZFP161.
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pubmed:affiliation |
Department of Molecular Biology, Keio University School of Medicine, 35 Shinanomachi, Shinjuku-ku, Tokyo 160-8582, Japan.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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