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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
1
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pubmed:dateCreated |
2005-1-4
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pubmed:abstractText |
Peptidylglycine-alpha-amidating monooxygenase (PAM) is a copper-dependent enzyme involved in peptide posttranslational activation. Dietary Cu deficiency (Cu-) was studied to determine if lower PAM activity was due to reduction in protein or cofactor limitation. PAM activity was lower in cardiac atria of Cu- rats than Cu-adequate (Cu+) rats and there was a 50% equivalent reduction in PAM protein. No reduction in Cu- rat midbrain PAM protein was detected although PAM activity was reduced 40%. In 12-day-old (P12) mouse pups derived from dams that began Cu deficiency on day 7 of gestation, there was a parallel reduction in brain PAM activity and protein of 40-50%. PAM mRNA levels assessed in atria and brains from Cu+ and Cu- rats and mice were not altered by dietary treatment, suggesting a posttranscriptional mechanism for lower PAM protein when Cu is limiting in the cell, perhaps due to enhanced apoprotein turnover.
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pubmed:grant |
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/15629125-10573542,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15629125-10719841,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15629125-11516174,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15629125-12488345,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15629125-12529325,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15629125-12878478,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15629125-15087449,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15629125-15113935,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15629125-1554368,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15629125-1575450,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15629125-2159243,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15629125-2325165,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15629125-3017953,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15629125-3996325,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15629125-5442275,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15629125-6312000,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15629125-6576381,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15629125-7568280,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15629125-7782897,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15629125-8246921,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15629125-8391562,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15629125-9185337,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15629125-9270721,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15629125-9599102,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15629125-98070,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15629125-9971741
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Feb
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pubmed:issn |
0003-9861
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
1
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pubmed:volume |
434
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
212-20
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:15629125-Animals,
pubmed-meshheading:15629125-Animals, Suckling,
pubmed-meshheading:15629125-Copper,
pubmed-meshheading:15629125-Female,
pubmed-meshheading:15629125-Gene Expression,
pubmed-meshheading:15629125-Heart Atria,
pubmed-meshheading:15629125-Male,
pubmed-meshheading:15629125-Mesencephalon,
pubmed-meshheading:15629125-Mice,
pubmed-meshheading:15629125-Mixed Function Oxygenases,
pubmed-meshheading:15629125-Multienzyme Complexes,
pubmed-meshheading:15629125-Pregnancy,
pubmed-meshheading:15629125-RNA, Messenger,
pubmed-meshheading:15629125-Rats,
pubmed-meshheading:15629125-Rats, Sprague-Dawley
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pubmed:year |
2005
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pubmed:articleTitle |
Peptidylglycine-alpha-amidating monooxygenase activity and protein are lower in copper-deficient rats and suckling copper-deficient mice.
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pubmed:affiliation |
Department of Biochemistry and Molecular Biology, University of Minnesota Medical School Duluth, 1035 University Drive, Duluth, MN 55812, USA. jprohask@d.umn.edu
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, U.S. Gov't, Non-P.H.S.
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