Linked Life Data

15627391

Source:http://linkedlifedata.com/resource/pubmed/id/15627391

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
12
pubmed:dateCreated
2005-1-3
pubmed:abstractText
Thirty-four primary hybridoma clones were prepared which expressed monoclonal antibodies to the Ca(2+)-binding protein recoverin. Among the resulting monoclonal antibodies, two Ca(2+)-dependent clones (mAb3 and mAb19) recognizing recoverin were detected by solid-phase immunoenzyme assay. In the presence of Ca(2+), antibodies of the mAb3 and mAb19 clones bound to recoverin several times better than in the absence of Ca(2+). The mAb3 and mAb19 antibodies recognized epitopes located inside the sequences Pro61-Met91 and Pro57-Tyr64 of the recoverin molecule, respectively. The possible mechanism of the Ca(2+)-dependent recognition of recoverin by the prepared monoclonal antibodies is discussed.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Dec
pubmed:issn
0006-2979
pubmed:author
pubmed:issnType
Print
pubmed:volume
69
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1360-4
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
2004
pubmed:articleTitle
Preparation and characteristics of Ca(2+)-dependent monoclonal antibodies to recoverin.
pubmed:affiliation
Department of Cell Signal Systems, Belozersky Institute of Physico-Chemical Biology, Lomonosov Moscow State University, Moscow 119992, Russia.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't