Source:http://linkedlifedata.com/resource/pubmed/id/15626895
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
6
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| pubmed:dateCreated |
2004-12-31
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| pubmed:abstractText |
Bactericidal/permeability-increasing protein (BPI) is an antineutrophil cytoplasmic autoantibody (ANCA) target antigen in inflammatory bowel disease (IBD). The aim of this study was to characterize binding regions of BPI-autoantibodies and to analyze their ability to block the antibiotic effect of BPI. Sera of 24 ulcerative colitis and Crohn's disease patients were examined in indirect immuno-fluorescence, ANCA enzyme-linked immunosorbent assay (ELISA), and by epitope mapping with 13mer peptides and Western blot for presence of BPI-autoantibodies. IgG preparations were used to determine inhibition of BPI's antimicrobial function by BPI-autoantibodies in a bacterial growth inhibition assay. BPI-autoantibodies were detected by ELISA in 18/24 patients. Epitope mapping and western blotting revealed an additional 3 patients with BPI-autoantibodies. IgG preparations of all patients with Crohn's disease and 9 of 12 ulcerative colitis patients could inhibit the antibiotic function of BPI in vitro as compared with healthy control subjects. Inhibiting BPI-autoantibodies correlated with extraintestinal manifestations, peripheral blood leukocyte counts, and anemia. BPI-autoantibodies recognizing the N-terminal portion were associated with greater mucosal damage and intestinal extent of disease. BPI is a frequent target antigen of autoantibodies in ulcerative colitis and Crohn's disease. Inhibition of the antibiotic function mediated by the N-terminal region of BPI by these autoantibodies may contribute to a proinflammatory environment in IBD patients.
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| pubmed:language |
eng
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| pubmed:journal | |
| pubmed:citationSubset |
IM
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| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Anti-Bacterial Agents,
http://linkedlifedata.com/resource/pubmed/chemical/Antibodies, Antineutrophil...,
http://linkedlifedata.com/resource/pubmed/chemical/Antimicrobial Cationic Peptides,
http://linkedlifedata.com/resource/pubmed/chemical/Blood Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Membrane Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/bactericidal permeability...
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| pubmed:status |
MEDLINE
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| pubmed:month |
Nov
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| pubmed:issn |
1078-0998
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| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
10
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
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| pubmed:pagination |
763-70
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| pubmed:dateRevised |
2006-11-15
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| pubmed:meshHeading |
pubmed-meshheading:15626895-Adolescent,
pubmed-meshheading:15626895-Adult,
pubmed-meshheading:15626895-Anti-Bacterial Agents,
pubmed-meshheading:15626895-Antibodies, Antineutrophil Cytoplasmic,
pubmed-meshheading:15626895-Antimicrobial Cationic Peptides,
pubmed-meshheading:15626895-Blood Bactericidal Activity,
pubmed-meshheading:15626895-Blood Proteins,
pubmed-meshheading:15626895-Blotting, Western,
pubmed-meshheading:15626895-Case-Control Studies,
pubmed-meshheading:15626895-Colitis, Ulcerative,
pubmed-meshheading:15626895-Crohn Disease,
pubmed-meshheading:15626895-Enzyme-Linked Immunosorbent Assay,
pubmed-meshheading:15626895-Escherichia coli,
pubmed-meshheading:15626895-Female,
pubmed-meshheading:15626895-Humans,
pubmed-meshheading:15626895-Male,
pubmed-meshheading:15626895-Membrane Proteins,
pubmed-meshheading:15626895-Middle Aged
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| pubmed:year |
2004
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| pubmed:articleTitle |
Autoantibodies against the bactericidal/permeability-increasing protein from inflammatory bowel disease patients can impair the antibiotic activity of bactericidal/permeability-increasing protein.
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| pubmed:affiliation |
Department of Rheumatology, University of Luebeck, Bad Bramstedt, Germany.
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| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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