Linked Life Data

15623547

Source:http://linkedlifedata.com/resource/pubmed/id/15623547

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
2005-1-19
pubmed:abstractText
Persistent human papillomavirus (HPV) type 16 and 18 infection can lead to pre-malignant and malignant diseases of the lower genital tract. Several lines of evidence suggest that T cell responses can control HPV infection. However, relative to other human viruses, strong effector memory T cell responses against HPV have been difficult to detect. We used an in vitro stimulation step prior to enzyme-linked immunospot assays to identify IFN-gamma-secreting T cells specific for HPV16 and 18 E6/E7 peptides. This allowed the detection of HPV-specific CD4+ T cells that were not evident in direct ex vivo assays. T cell responses against HPV16 or 18 peptides were detected in healthy volunteers (7/9) and patients with lower genital tract neoplasia (10/20). Importantly, this assay allowed tracking of vaccine-induced T cell responses in nine patients, following inoculation with a live recombinant vaccinia virus (HPV16 and 18 E6/E7, TA-HPV). Novel vaccine-induced T cell responses were demonstrated in five patients, but no clinical responses (lesion regressions) were seen. For one vaccinated patient, the T cell response was mapped to a single dominant HPV18 E7 epitope and this response was sustained for >3 years. Our data suggest that systemic memory T cells against HPV16 and 18, induced naturally or by TA-HPV vaccination, are relatively rare. Nevertheless, the assay system developed allowed estimation of magnitude, epitope specificity, and longevity of vaccine-induced CD4+ T cell responses. This will be useful for vaccine design and measurement of immunological endpoints in clinical trials.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Feb
pubmed:issn
0953-8178
pubmed:author
pubmed:issnType
Print
pubmed:volume
17
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
167-76
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed-meshheading:15623547-Adult, pubmed-meshheading:15623547-Amino Acid Sequence, pubmed-meshheading:15623547-CD4-Positive T-Lymphocytes, pubmed-meshheading:15623547-DNA-Binding Proteins, pubmed-meshheading:15623547-Epitopes, T-Lymphocyte, pubmed-meshheading:15623547-Female, pubmed-meshheading:15623547-Humans, pubmed-meshheading:15623547-Immunoenzyme Techniques, pubmed-meshheading:15623547-Immunologic Memory, pubmed-meshheading:15623547-Male, pubmed-meshheading:15623547-Middle Aged, pubmed-meshheading:15623547-Molecular Sequence Data, pubmed-meshheading:15623547-Oncogene Proteins, Viral, pubmed-meshheading:15623547-Papillomaviridae, pubmed-meshheading:15623547-Papillomavirus Infections, pubmed-meshheading:15623547-Papillomavirus Vaccines, pubmed-meshheading:15623547-Peptides, pubmed-meshheading:15623547-Uterine Cervical Dysplasia, pubmed-meshheading:15623547-Vaccines, Synthetic, pubmed-meshheading:15623547-Viral Vaccines, pubmed-meshheading:15623547-Vulvar Neoplasms
pubmed:year
2005
pubmed:articleTitle
Epitope specificity and longevity of a vaccine-induced human T cell response against HPV18.
pubmed:affiliation
Section of Infection and Immunity, Wales College of Medicine, Cardiff University, Henry Wellcome Research Building, Heath Park, Cardiff CF14 4XX, UK.
pubmed:publicationType
Journal Article, Comparative Study, Research Support, Non-U.S. Gov't, Evaluation Studies