Linked Life Data

15623426

Source:http://linkedlifedata.com/resource/pubmed/id/15623426

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
2004-12-29
pubmed:abstractText
It is well known that repetitive ischemia followed by reperfusion (four cycles of 5 min of ischemia and 10 min of reperfusion) demonstrates protective effect against subsequent severe ischemic insult, known as ischemic preconditioning (PC). This phenomenon causes reduction in oxidative DNA damage, infarct size, and the extent of apoptotic cell death, leading to adaptation on functional recovery. The involvement of DNA-repair mechanisms in PC has not been well studied. We utilized the antibody-array technique to identify DNA-repair proteins that were upregulated by ischemic PC in the permanent left anterior coronary artery occlusion myocardial infarction (MI) model. The antibody-array system enabled us to identify three double-strand-break-repair proteins--Rad50, DNA topoisomerase I, Ku80--that were upregulated and might be involved in cell-survival processes during adaptation. With Western blot analysis, we found no significant difference in Ku80 protein expression between preconditioned and control groups after MI. Therefore, this report focuses on the overexpression of Rad50 and DNA topoisomerase and proposes that the DNA-repair mechanism in the permanent left anterior descending coronary artery (LAD) occlusion model involves these two proteins.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jan
pubmed:issn
0022-2828
pubmed:author
pubmed:issnType
Print
pubmed:volume
38
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
99-102
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed:year
2005
pubmed:articleTitle
Antibody-array technique reveals overexpression of important DNA-repair proteins during cardiac ischemic preconditioning.
pubmed:affiliation
Molecular Cardiology Laboratory, Department of Surgery, University of Connecticut Health Center, School of Medicine, Farmington, CT 06030-1110, USA.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, U.S. Gov't, Non-P.H.S.