Source:http://linkedlifedata.com/resource/pubmed/id/15620461
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
1
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| pubmed:dateCreated |
2004-12-28
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| pubmed:abstractText |
The nonclassic human leukocyte antigen (HLA)-DM molecules have been proved to positively regulate antigen presentation in classic antigen-presenting cells, whereas in B lymphocytes HLA-DO have been identified as negative regulators of the process. The present report examines whether the negative expression of classic class II molecules in trophoblasts implies negative regulation by HLA-DO. It was revealed by immunofluorescence, confocal microscopy, and subcellular fractionation techniques that human trophoblasts, although not expressing any surface HLA-DR antigens, constitutively express intracellular HLA-DR, HLA-DO, and CD74, but not HLA-DM. Administration of interferon-gamma to the cell culture increased HLA-DR and CD74, induced HLA-DM, but did not alter the expression of HLA-DO and induced HLA-DR release from the cells. These results were confirmed by reverse transcriptase-polymerase chain reaction analysis except that HLA-DM mRNA was detected in control cells, indicating a posttranscriptional regulation. Under the same experimental conditions, human monocytes/macrophages were not expressing intracellular HLA-DO while exhibiting significant levels of HLA-DR, HLA-DM, and CD74. The results presented here reveal for the first time expression of HLA-DO in trophoblasts, which can be of great importance in maintaining the class II-negative state in these cells and consequently protecting the fetus from maternal immune attack.
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| pubmed:language |
eng
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| pubmed:journal | |
| pubmed:citationSubset |
IM
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| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, Differentiation...,
http://linkedlifedata.com/resource/pubmed/chemical/HLA-D Antigens,
http://linkedlifedata.com/resource/pubmed/chemical/HLA-DM antigens,
http://linkedlifedata.com/resource/pubmed/chemical/HLA-DO antigens,
http://linkedlifedata.com/resource/pubmed/chemical/HLA-DR Antigens,
http://linkedlifedata.com/resource/pubmed/chemical/Histocompatibility Antigens Class II,
http://linkedlifedata.com/resource/pubmed/chemical/Interferon-gamma,
http://linkedlifedata.com/resource/pubmed/chemical/Recombinant Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/invariant chain
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| pubmed:status |
MEDLINE
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| pubmed:month |
Jan
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| pubmed:issn |
0198-8859
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| pubmed:author | |
| pubmed:issnType |
Print
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| pubmed:volume |
66
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| pubmed:owner |
NLM
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| pubmed:authorsComplete |
Y
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| pubmed:pagination |
43-55
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| pubmed:dateRevised |
2011-11-17
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| pubmed:meshHeading |
pubmed-meshheading:15620461-Animals,
pubmed-meshheading:15620461-Antigen-Presenting Cells,
pubmed-meshheading:15620461-Antigens, Differentiation, B-Lymphocyte,
pubmed-meshheading:15620461-Cell Line,
pubmed-meshheading:15620461-Cell Membrane,
pubmed-meshheading:15620461-Cytoplasm,
pubmed-meshheading:15620461-Female,
pubmed-meshheading:15620461-Fluorescent Antibody Technique,
pubmed-meshheading:15620461-HLA-D Antigens,
pubmed-meshheading:15620461-HLA-DR Antigens,
pubmed-meshheading:15620461-Histocompatibility Antigens Class II,
pubmed-meshheading:15620461-Humans,
pubmed-meshheading:15620461-Interferon-gamma,
pubmed-meshheading:15620461-Macrophages,
pubmed-meshheading:15620461-Mice,
pubmed-meshheading:15620461-Monocytes,
pubmed-meshheading:15620461-Recombinant Proteins,
pubmed-meshheading:15620461-Trophoblasts
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| pubmed:year |
2005
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| pubmed:articleTitle |
Constitutive intracellular expression of human leukocyte antigen (HLA)-DO and HLA-DR but not HLA-DM in trophoblast cells.
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| pubmed:affiliation |
Department of Biology, Laboratory of Immunology, University of Crete, Heraklion, Crete, Greece.
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| pubmed:publicationType |
Journal Article
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