Linked Life Data

15619909

Source:http://linkedlifedata.com/resource/pubmed/id/15619909

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
2004-12-28
pubmed:abstractText
The partial envelope (E) protein gene of Dengue virus type 4 (DV4), derived from the serum of a patient with dengue fever in an epidemic during October 1993 in Guangdong Province of China, was cloned and sequenced. DV RNA was converted to cDNA by reverse transcription with random primers before the polymerase chain reaction using DV4 specific primers was performed. The amplified product of 421bp was subsequently filled in 3' recessed ends, isolated, purified and inserted into pUC18 and pUC19 plasmid vector. Their sequences were determined by dideoxy nucleotide chain termination method. A comparison between DV4 Chinese strain and several other previously reported strains shows the homology to be 93.72% for the same dengue serotype, 61.26-64.40% for the other dengue serotypes, only 40.31% for Japanese encephalitis virus, which belongs to the member of flavivirus family. A conserved sequence with 12 amino acids observed from deduced protein sequence probably represents an essential functional element among flavivirus family.
pubmed:language
chi
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Mar
pubmed:issn
1003-9279
pubmed:author
pubmed:issnType
Print
pubmed:volume
11
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
62-5
pubmed:meshHeading
pubmed:year
1997
pubmed:articleTitle
[The cloning and sequence analysis of cDNA of the partial E genomic region of Dengue type 4 virus from a Chinese patient].
pubmed:affiliation
Department of Infectious Diseaes, Sun Yatsen University of Medical Sciences, Guangzhou 510630.
pubmed:publicationType
Journal Article, English Abstract, Research Support, Non-U.S. Gov't