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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
3
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pubmed:dateCreated |
2005-2-15
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pubmed:abstractText |
Keratinocyte apoptosis induced by UV radiation is a major protective mechanism from skin photocarcinogenesis. The induction of apoptosis by UV radiation, as well as a variety of genotoxic stimuli, involves the activation of PKC-delta by caspase-3-mediated cleavage in its hinge region, thus generating a constitutively active catalytic fragment. To determine the role of PKC-delta cleavage in UV apoptosis signaling, we introduced a caspase-resistant PKC-delta mutant (D330A) into human keratinocytes by retrovirus transduction. Overexpression of PKC-delta(D330A) protected keratinocytes from UV-induced apoptosis and enhanced long-term survival. PKC-delta(D330A) partially prevented the release of cytochrome c from the mitochondria and the loss of Mcl-1, a key antiapoptotic protein downregulated during UV apoptosis. Thus, the cleavage and activation of PKC-delta are critical components of UV-induced apoptosis in human keratinocytes, and the inactivation of PKC-delta can promote the survival of keratinocytes exposed to UV radiation.
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pubmed:grant |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/CASP3 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Caspase 3,
http://linkedlifedata.com/resource/pubmed/chemical/Caspases,
http://linkedlifedata.com/resource/pubmed/chemical/Cytochromes c,
http://linkedlifedata.com/resource/pubmed/chemical/Neoplasm Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/PRKCD protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Protein Kinase C,
http://linkedlifedata.com/resource/pubmed/chemical/Protein Kinase C-delta,
http://linkedlifedata.com/resource/pubmed/chemical/Proto-Oncogene Proteins c-bcl-2,
http://linkedlifedata.com/resource/pubmed/chemical/myeloid cell leukemia sequence 1...
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pubmed:status |
MEDLINE
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pubmed:month |
Mar
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pubmed:issn |
1350-9047
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
12
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
224-32
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pubmed:dateRevised |
2009-11-19
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pubmed:meshHeading |
pubmed-meshheading:15618968-Apoptosis,
pubmed-meshheading:15618968-Caspase 3,
pubmed-meshheading:15618968-Caspases,
pubmed-meshheading:15618968-Catalytic Domain,
pubmed-meshheading:15618968-Cell Survival,
pubmed-meshheading:15618968-Cells, Cultured,
pubmed-meshheading:15618968-Cytochromes c,
pubmed-meshheading:15618968-Down-Regulation,
pubmed-meshheading:15618968-Enzyme Activation,
pubmed-meshheading:15618968-Flow Cytometry,
pubmed-meshheading:15618968-Humans,
pubmed-meshheading:15618968-Keratinocytes,
pubmed-meshheading:15618968-Membrane Potentials,
pubmed-meshheading:15618968-Mitochondria,
pubmed-meshheading:15618968-Mutation,
pubmed-meshheading:15618968-Neoplasm Proteins,
pubmed-meshheading:15618968-Protein Kinase C,
pubmed-meshheading:15618968-Protein Kinase C-delta,
pubmed-meshheading:15618968-Protein Transport,
pubmed-meshheading:15618968-Proto-Oncogene Proteins c-bcl-2,
pubmed-meshheading:15618968-Ultraviolet Rays
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pubmed:year |
2005
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pubmed:articleTitle |
A caspase-resistant mutant of PKC-delta protects keratinocytes from UV-induced apoptosis.
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pubmed:affiliation |
Division of Molecular and Cellular Biochemistry, Cardinal Bernardin Cancer Center, Loyola University Medical Center, Maywood, IL 60153, USA.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, N.I.H., Extramural
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