15618318

Source:http://linkedlifedata.com/resource/pubmed/id/15618318

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Predicate	Object
rdf:type	pubmed:Citation
lifeskim:mentions	umls-concept:C0004927, umls-concept:C0005537, umls-concept:C0015350, umls-concept:C0016030, umls-concept:C0205276, umls-concept:C0486616, umls-concept:C0851285, umls-concept:C2347299
pubmed:issue	5
pubmed:dateCreated	2005-4-14
pubmed:abstractText	The extracellular matrix (ECM) provides the principal means by which mechanical information is communicated between tissue and cellular levels of function. These mechanical signals play a central role in controlling cell fate and establishing tissue structure and function. However, little is known regarding the mechanisms by which specific structural and mechanical properties of the ECM influence its interaction with cells, especially within a tissuelike context. This lack of knowledge precludes formulation of biomimetic microenvironments for effective tissue repair and replacement. The present study determined the role of collagen fibril density in regulating local cell-ECM biomechanics and fundamental fibroblast behavior. The model system consisted of fibroblasts seeded within collagen ECMs with controlled microstructure. Confocal microscopy was used to collect multidimensional images of both ECM microstructure and specific cellular characteristics. From these images temporal changes in three-dimensional cell morphology, time- and space-dependent changes in the three-dimensional local strain state of a cell and its ECM, and spatial distribution of beta1-integrin were quantified. Results showed that fibroblasts grown within high-fibril-density ECMs had decreased length-to-height ratios, increased surface areas, and a greater number of projections. Furthermore, fibroblasts within low-fibril-density ECMs reorganized their ECM to a greater extent, and it appeared that beta1-integrin localization was related to local strain and ECM remodeling events. Finally, fibroblast proliferation was enhanced in low-fibril-density ECMs. Collectively, these results are significant because they provide new insight into how specific physical properties of a cell's ECM microenvironment contribute to tissue remodeling events in vivo and to the design and engineering of functional tissue replacements.
pubmed:grant	http://linkedlifedata.com/resource/pubmed/grant/1R01EB000165
pubmed:language	eng
pubmed:journal	http://linkedlifedata.com/resource/pubmed/journal/8502536
pubmed:citationSubset	IM
pubmed:status	MEDLINE
pubmed:month	May
pubmed:issn	8750-7587
pubmed:author	pubmed-author:KokiniKK, pubmed-author:PizzoA MAM, pubmed-author:VaughnL CLC, pubmed-author:Voytik-HarbinS LSL, pubmed-author:WaisnerB ZBZ
pubmed:issnType	Print
pubmed:volume	98
pubmed:owner	NLM
pubmed:authorsComplete	Y
pubmed:pagination	1909-21
pubmed:dateRevised	2007-11-14
pubmed:meshHeading	pubmed-meshheading:15618318-Biomechanics, pubmed-meshheading:15618318-Cells, Cultured, pubmed-meshheading:15618318-Extracellular Matrix, pubmed-meshheading:15618318-Fibroblasts, pubmed-meshheading:15618318-Humans, pubmed-meshheading:15618318-Surface Properties
pubmed:year	2005
pubmed:articleTitle	Extracellular matrix (ECM) microstructural composition regulates local cell-ECM biomechanics and fundamental fibroblast behavior: a multidimensional perspective.
pubmed:affiliation	School of Mechanical Engineering, School of Veterinary Medicine, Purdue University, West Lafayette, IN 47907, USA.
pubmed:publicationType	Journal Article, Comparative Study, Research Support, U.S. Gov't, P.H.S., Research Support, U.S. Gov't, Non-P.H.S., Research Support, Non-U.S. Gov't, Research Support, N.I.H., Extramural