Linked Life Data

15618165

Source:http://linkedlifedata.com/resource/pubmed/id/15618165

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
2004-12-24
pubmed:abstractText
A 42-kDa fragment from the C terminus of major merozoite surface protein 1 (MSP1) is among the leading malaria vaccine candidates that target infection by asexual erythrocytic-stage malaria parasites. The MSP1(42) gene fragment from the Vietnam-Oak Knoll (FVO) strain of Plasmodium falciparum was expressed as a soluble protein in Escherichia coli and purified according to good manufacturing practices. This clinical-grade recombinant protein retained some important elements of correct structure, as it was reactive with several functional, conformation-dependent monoclonal antibodies raised against P. falciparum malaria parasites, it induced antibodies (Abs) that were reactive to parasites in immunofluorescent Ab tests, and it induced strong growth and invasion inhibitory antisera in New Zealand White rabbits. The antigen quality was further evaluated by vaccinating Aotus nancymai monkeys and challenging them with homologous P. falciparum FVO erythrocytic-stage malaria parasites. The trial included two control groups, one vaccinated with the sexual-stage-specific antigen of Plasmodium vivax, Pvs25, as a negative control, and the other vaccinated with baculovirus-expressed MSP1(42) (FVO) as a positive control. Enzyme-linked immunosorbent assay (ELISA) Ab titers induced by E. coli MSP1(42) were significantly higher than those induced by the baculovirus-expressed antigen. None of the six monkeys that were vaccinated with the E. coli MSP1(42) antigen required treatment for uncontrolled parasitemia, but two required treatment for anemia. Protective immunity in these monkeys correlated with the ELISA Ab titer against the p19 fragment and the epidermal growth factor (EGF)-like domain 2 fragment of MSP1(42), but not the MSP1(42) protein itself or the EGF-like domain 1 fragment. Soluble MSP1(42) (FVO) expressed in E. coli offers excellent promise as a component of a vaccine against erythrocytic-stage falciparum malaria.
pubmed:grant
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/15618165-10205793, http://linkedlifedata.com/resource/pubmed/commentcorrection/15618165-10339410, http://linkedlifedata.com/resource/pubmed/commentcorrection/15618165-10556701, http://linkedlifedata.com/resource/pubmed/commentcorrection/15618165-11083773, http://linkedlifedata.com/resource/pubmed/commentcorrection/15618165-11179324, http://linkedlifedata.com/resource/pubmed/commentcorrection/15618165-11292349, http://linkedlifedata.com/resource/pubmed/commentcorrection/15618165-11413195, http://linkedlifedata.com/resource/pubmed/commentcorrection/15618165-11530352, http://linkedlifedata.com/resource/pubmed/commentcorrection/15618165-11530353, http://linkedlifedata.com/resource/pubmed/commentcorrection/15618165-11752405, http://linkedlifedata.com/resource/pubmed/commentcorrection/15618165-12125145, http://linkedlifedata.com/resource/pubmed/commentcorrection/15618165-12125152, http://linkedlifedata.com/resource/pubmed/commentcorrection/15618165-12438375, http://linkedlifedata.com/resource/pubmed/commentcorrection/15618165-12742586, http://linkedlifedata.com/resource/pubmed/commentcorrection/15618165-14638762, http://linkedlifedata.com/resource/pubmed/commentcorrection/15618165-1624802, http://linkedlifedata.com/resource/pubmed/commentcorrection/15618165-1694225, http://linkedlifedata.com/resource/pubmed/commentcorrection/15618165-1741018, http://linkedlifedata.com/resource/pubmed/commentcorrection/15618165-1775158, http://linkedlifedata.com/resource/pubmed/commentcorrection/15618165-2433334, http://linkedlifedata.com/resource/pubmed/commentcorrection/15618165-2437453, http://linkedlifedata.com/resource/pubmed/commentcorrection/15618165-3049134, http://linkedlifedata.com/resource/pubmed/commentcorrection/15618165-3295686, http://linkedlifedata.com/resource/pubmed/commentcorrection/15618165-3886646, http://linkedlifedata.com/resource/pubmed/commentcorrection/15618165-6151681, http://linkedlifedata.com/resource/pubmed/commentcorrection/15618165-6350871, http://linkedlifedata.com/resource/pubmed/commentcorrection/15618165-6725950, http://linkedlifedata.com/resource/pubmed/commentcorrection/15618165-7516416, http://linkedlifedata.com/resource/pubmed/commentcorrection/15618165-7694147, http://linkedlifedata.com/resource/pubmed/commentcorrection/15618165-8015856, http://linkedlifedata.com/resource/pubmed/commentcorrection/15618165-8074257, http://linkedlifedata.com/resource/pubmed/commentcorrection/15618165-8515771, http://linkedlifedata.com/resource/pubmed/commentcorrection/15618165-8529111, http://linkedlifedata.com/resource/pubmed/commentcorrection/15618165-8557348, http://linkedlifedata.com/resource/pubmed/commentcorrection/15618165-9353014, http://linkedlifedata.com/resource/pubmed/commentcorrection/15618165-9362529
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jan
pubmed:issn
0019-9567
pubmed:author
pubmed:issnType
Print
pubmed:volume
73
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
287-97
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
pubmed:year
2005
pubmed:articleTitle
The clinical-grade 42-kilodalton fragment of merozoite surface protein 1 of Plasmodium falciparum strain FVO expressed in Escherichia coli protects Aotus nancymai against challenge with homologous erythrocytic-stage parasites.
pubmed:affiliation
Department of Immunology, Walter Reed Army Institute of Research, 503 Robert Grant Ave., Silver Spring, MD 20910, USA.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, U.S. Gov't, Non-P.H.S.