Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
2004-12-24
pubmed:abstractText
The organophosphate insecticide mevinphos (Mev) acts on the rostral ventrolateral medulla (RVLM), where sympathetic vasomotor tone originates, to elicit phasic cardiovascular responses via nitric oxide (NO) generated by NO synthase (NOS) I and II. We evaluated the contribution of soluble guanylyl cyclase (sGC)/cyclic guanosine monophosphate (cGMP)/protein kinase G (PKG) cascade and peroxynitrite in this process. PKG expression in ventrolateral medulla of Sprague-Dawley rats manifested an increase during the sympathoexcitatory phase (Phase I) of cardiovascular responses induced by microinjection of Mev bilaterally into the RVLM that was antagonized by co-administration of 7-nitroindazole or Nomega-propyl-L-arginine, two selective NOS I inhibitors or 1-H-[1,2,4]oxadiaolo[4,3-a]quinoxalin-1-one (ODQ), a selective sGC antagonist. Co-microinjection of ODQ or two PKG inhibitors, KT5823 or Rp-8-Br-cGMPS, also blunted the Mev-elicited sympathoexcitatory effects. However, the increase in nitrotyrosine, a marker for peroxynitrite, and the sympathoinhibitory circulatory actions during Phase II Mev intoxication were antagonized by co-administration of S-methylisothiourea, a selective NOS II inhibitor, Mn(III)-tetrakis-(4-benzoic acid) porphyrin, a superoxide dismutase mimetic, 5,10,15,20-tetrakis-N-methyl-4'-pyridyl)-porphyrinato iron (III), a peroxynitrite decomposition catalyst, or L-cysteine, a peroxynitrite scavenger. We conclude that sGC/cGMP/PKG cascade and peroxynitrite formation may participate in Mev-induced phasic cardiovascular responses as signals downstream to NO generated respectively by NOS I and II in the RVLM.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
http://linkedlifedata.com/resource/pubmed/chemical/3-nitrotyrosine, http://linkedlifedata.com/resource/pubmed/chemical/Cholinesterase Inhibitors, http://linkedlifedata.com/resource/pubmed/chemical/Cyclic GMP, http://linkedlifedata.com/resource/pubmed/chemical/Cyclic GMP-Dependent Protein Kinases, http://linkedlifedata.com/resource/pubmed/chemical/Mevinphos, http://linkedlifedata.com/resource/pubmed/chemical/Nerve Tissue Proteins, http://linkedlifedata.com/resource/pubmed/chemical/Nitric Oxide, http://linkedlifedata.com/resource/pubmed/chemical/Nitric Oxide Synthase, http://linkedlifedata.com/resource/pubmed/chemical/Nitric Oxide Synthase Type I, http://linkedlifedata.com/resource/pubmed/chemical/Nitric Oxide Synthase Type II, http://linkedlifedata.com/resource/pubmed/chemical/Nos1 protein, rat, http://linkedlifedata.com/resource/pubmed/chemical/Peroxynitrous Acid, http://linkedlifedata.com/resource/pubmed/chemical/Superoxides, http://linkedlifedata.com/resource/pubmed/chemical/Tyrosine
pubmed:status
MEDLINE
pubmed:month
Jan
pubmed:issn
0028-3908
pubmed:author
pubmed:issnType
Print
pubmed:volume
48
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
161-72
pubmed:dateRevised
2009-11-19
pubmed:meshHeading
pubmed-meshheading:15617736-Animals, pubmed-meshheading:15617736-Blood Pressure, pubmed-meshheading:15617736-Blotting, Western, pubmed-meshheading:15617736-Cardiovascular Physiological Phenomena, pubmed-meshheading:15617736-Cholinesterase Inhibitors, pubmed-meshheading:15617736-Cyclic GMP, pubmed-meshheading:15617736-Cyclic GMP-Dependent Protein Kinases, pubmed-meshheading:15617736-Heart Rate, pubmed-meshheading:15617736-Male, pubmed-meshheading:15617736-Medulla Oblongata, pubmed-meshheading:15617736-Mevinphos, pubmed-meshheading:15617736-Microinjections, pubmed-meshheading:15617736-Nerve Tissue Proteins, pubmed-meshheading:15617736-Nitric Oxide, pubmed-meshheading:15617736-Nitric Oxide Synthase, pubmed-meshheading:15617736-Nitric Oxide Synthase Type I, pubmed-meshheading:15617736-Nitric Oxide Synthase Type II, pubmed-meshheading:15617736-Peroxynitrous Acid, pubmed-meshheading:15617736-Rats, pubmed-meshheading:15617736-Spectrum Analysis, pubmed-meshheading:15617736-Superoxides, pubmed-meshheading:15617736-Time Factors, pubmed-meshheading:15617736-Tyrosine
pubmed:year
2005
pubmed:articleTitle
Phasic cardiovascular responses to mevinphos are mediated through differential activation of cGMP/PKG cascade and peroxynitrite via nitric oxide generated in the rat rostral ventrolateral medulla by NOS I and II isoforms.
pubmed:affiliation
Department of Medical Education and Research, Kaohsiung Veterans General Hospital, Kaohsiung 813, Taiwan, Republic of China.
pubmed:publicationType
Journal Article, Comparative Study, Research Support, U.S. Gov't, Non-P.H.S., Research Support, Non-U.S. Gov't