Linked Life Data

15611234

Source:http://linkedlifedata.com/resource/pubmed/id/15611234

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
2004-12-21
pubmed:abstractText
Mast cells rely on Ca(2+) signaling to initiate activation programs leading to release of proinflammatory mediators. The interplay between Ca(2+) release from internal stores and Ca(2+) entry through store-operated Ca(2+) channels has been extensively studied. Using rat basophilic leukemia (RBL) mast cells and murine bone marrow-derived mast cells, we examine the role of Na(+)/Ca(2+) exchangers. Calcium imaging experiments and patch clamp current recordings revealed both K(+)-independent and K(+)-dependent components of Na(+)/Ca(2+) exchange. Northern blot analysis indicated the predominant expression of the K(+)-dependent sodium-calcium exchanger NCKX3. Transcripts of the exchangers NCX3 and NCKX1 were additionally detected in RBL cells with RT-PCR. The Ca(2+) clearance via Na(+)/Ca(2+) exchange represented approximately 50% of the total clearance when Ca(2+) signals reached levels > or =200 nM. Ca(2+) signaling and store-operated Ca(2+) entry were strongly reduced by inverting the direction of Na(+)/Ca(2+) exchange, indicating that Na(+)/Ca(2+) exchangers normally extrude Ca(2+) ions from cytosol and prevent the Ca(2+)-dependent inactivation of store-operated Ca(2+) channels. Working in the Ca(2+) efflux mode, Na(+)/Ca(2+) exchangers such as NCKX3 and NCX3 might, therefore, play a role in the Ag-induced mast cell activation by controlling the sustained phase of Ca(2+) mobilization.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
AIM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jan
pubmed:issn
0022-1767
pubmed:author
pubmed:issnType
Print
pubmed:day
1
pubmed:volume
174
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
119-30
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
2005
pubmed:articleTitle
Modulation of Ca2+ signaling by Na+/Ca2+ exchangers in mast cells.
pubmed:affiliation
Pharmakologie und Toxikologie, Universität des Saarlandes, D-66421 Homburg, Germany.
pubmed:publicationType
Journal Article, In Vitro