15607822

pubmed:Citation

5

The transcription factor LKLF (lung Krüppel-like factor) is expressed at high levels in quiescent CD4+ and CD8+ T lymphocytes and provides an important role in establishment and maintenance of the quiescent state. To identify LKLF-regulated genes, we performed microarray analysis using an established Jurkat T cell line containing a tetracycline-inducible LKLF. LKLF induction in this Jurkat T cell line generates a quiescent phenotype that resembles memory CD4+ T lymphocytes. We found that LKLF induction in Jurkat cells resulted in up-regulation (>1.5-fold) of about 100 mRNAs, while it repressed (>1.5-fold) a similar number of mRNAs. A striking feature of the LKLF-stimulated mRNAs was that a significant number of them encode cell surface proteins or proteins implicated in initiating and propagating cellular signaling cascades. The data suggests that LKLF may establish a phenotype that primes quiescent cells for responses to specific extracellular stimuli. The mRNAs encoding CDw52, IL-10R alpha and paxillin were among the most highly induced transcripts in Jurkat T cells, and we observed that the encoded proteins are down-regulated following activation of quiescent CD4+ T cells isolated from healthy blood donors. We also examined whether LKLF-induced quiescence in Jurkat cells could silence transcription of integrated HIV-1 proviruses. We found however that LKLF-induced quiescence is not sufficient to repress expression of HIV-1 proviruses in Jurkat T cells, suggesting that the HIV-1 provirus is resistant to LKLF-regulated quiescence.

eng

IM

MEDLINE

0161-5890

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NLM

627-41

pubmed-meshheading:15607822-Antigens, CD, pubmed-meshheading:15607822-Antigens, Neoplasm, pubmed-meshheading:15607822-Base Sequence, pubmed-meshheading:15607822-CD4-Positive T-Lymphocytes, pubmed-meshheading:15607822-Cytoskeletal Proteins, pubmed-meshheading:15607822-DNA, pubmed-meshheading:15607822-G0 Phase, pubmed-meshheading:15607822-Gene Expression Regulation, pubmed-meshheading:15607822-Glycoproteins, pubmed-meshheading:15607822-HIV Long Terminal Repeat, pubmed-meshheading:15607822-Humans, pubmed-meshheading:15607822-Jurkat Cells, pubmed-meshheading:15607822-Kruppel-Like Transcription Factors, pubmed-meshheading:15607822-Oligonucleotide Array Sequence Analysis, pubmed-meshheading:15607822-Paxillin, pubmed-meshheading:15607822-Phosphoproteins, pubmed-meshheading:15607822-RNA, Messenger, pubmed-meshheading:15607822-Receptors, Interleukin, pubmed-meshheading:15607822-Receptors, Interleukin-10, pubmed-meshheading:15607822-Trans-Activators

Identification of LKLF-regulated genes in quiescent CD4+ T lymphocytes.

Journal Article, In Vitro, Research Support, U.S. Gov't, P.H.S.