Linked Life Data

15607800

Source:http://linkedlifedata.com/resource/pubmed/id/15607800

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
4
pubmed:dateCreated
2004-12-20
pubmed:abstractText
The capability of NK lymphocytes to kill tumor cells depends on different receptors/ligands interactions. In order to identify the cellular ligands recognized by "orphan" triggering receptors, mice were immunized with NK susceptible target cells. mAbs were selected that inhibited NK cytotoxicity and recognized two different molecules of 70 and 60-65 kDa. Tryptic digestion and mass spectra analysis of purified proteins identified these molecules as PVR and Nectin-2, respectively. PVR-Fc and Nectin-2-Fc chimeric molecules stained COS-7 cells expressing the DNAM-1 activating receptor and conversely, PVR and Nectin-2 CHO-K cell transfectants were stained by DNAM-1-Fc. Thus, both PVR and Nectin-2 represent specific ligands for DNAM-1. Importantly, the specific interaction between DNAM-1 (in NK cells) and PVR or Nectin-2 (in target cells) enhanced the NK-mediated lysis of tumor cells that was downregulated by mAb-mediated masking of the receptor or its ligands.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Feb
pubmed:issn
0161-5890
pubmed:author
pubmed:issnType
Print
pubmed:volume
42
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
463-9
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed-meshheading:15607800-Animals, pubmed-meshheading:15607800-Antibodies, Monoclonal, pubmed-meshheading:15607800-Antigens, Differentiation, T-Lymphocyte, pubmed-meshheading:15607800-COS Cells, pubmed-meshheading:15607800-Cell Adhesion Molecules, pubmed-meshheading:15607800-Cercopithecus aethiops, pubmed-meshheading:15607800-Cytotoxicity, Immunologic, pubmed-meshheading:15607800-Humans, pubmed-meshheading:15607800-Immunoglobulin Fc Fragments, pubmed-meshheading:15607800-Killer Cells, Natural, pubmed-meshheading:15607800-Ligands, pubmed-meshheading:15607800-Membrane Proteins, pubmed-meshheading:15607800-Mice, pubmed-meshheading:15607800-Neoplasms, pubmed-meshheading:15607800-Peptide Mapping, pubmed-meshheading:15607800-Receptors, Virus, pubmed-meshheading:15607800-Recombinant Fusion Proteins
pubmed:year
2005
pubmed:articleTitle
PVR (CD155) and Nectin-2 (CD112) as ligands of the human DNAM-1 (CD226) activating receptor: involvement in tumor cell lysis.
pubmed:affiliation
Istituto Nazionale per la Ricerca sul Cancro, L.go R. Benzi 10, 16132 Genova, Italy. daniela.pende@istge.it
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't