Linked Life Data

15607425

Source:http://linkedlifedata.com/resource/pubmed/id/15607425

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
2004-12-20
pubmed:abstractText
Members of the Pumilio and DAZL family of RNA binding proteins are required for germ cell development in Drosophila, Xenopus, and Caenorhabditis elegans. Here, we report identification and characterization of RNA sequences to which PUM2 and DAZL bind. We established that human PUM2 specifically recognized the Drosophila Pumilio RNA target (the NRE or Nanos regulator element sequence); single nucleotide changes in the NRE abolished PUM2 binding. Then, we used coimmunoprecipitation to isolate human transcripts specifically bound by PUM2 and DAZL and subsequently identified those that contain NRE-like sequence elements. We confirmed that the interacting proteins, PUM2 and DAZL, are capable of binding the same RNA target and further characterized mRNA sequences bound by both proteins in the 3'UTR of human SDAD1 mRNA. Taken together, the results define sequences to which these germ cell-specific RNA binding proteins may bind to promote germ cell development.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jan
pubmed:issn
0888-7543
pubmed:author
pubmed:issnType
Print
pubmed:volume
85
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
92-105
pubmed:dateRevised
2008-10-16
pubmed:meshHeading
pubmed:year
2005
pubmed:articleTitle
Identification and characterization of RNA sequences to which human PUMILIO-2 (PUM2) and deleted in Azoospermia-like (DAZL) bind.
pubmed:affiliation
Program in Human Embryonic Stem Cell Biology, Center for Reproductive Sciences, Department of Obstetrics, Gynecology, and Reproductive Sciences, University of California at San Francisco, San Francisco, CA 94143-0556, USA.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't, Research Support, N.I.H., Extramural