Source:http://linkedlifedata.com/resource/pubmed/id/15605987
Switch to
| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
4
|
| pubmed:dateCreated |
2004-12-20
|
| pubmed:abstractText |
The distinction of astrocytomas and oligodendrogliomas, mainly pilocytic astrocytomas (PILOs) from infiltrating astrocytomas and oligodendrogliomas (ODs), and high-grade oligodendrogliomas from glioblastomas (GBMs), poses a serious clinical problem. There is no useful immunohistochemical (IHC) marker to differentiate these gliomas, and sometimes the differential diagnosis between them is arbitrary. We identified galectin-3 (Gal-3) as a possible tool to differentiate them based on gene expression profiles of GBMs. We confirmed the differential expression in 45 surgical samples (thirteen GBMs; seven PILOs; 5 grade II ODs; 5 anaplastic oligodendrogliomas [AODs], including 2 Oligo-astrocytomas; 8 diffuse astrocytomas [ASTs], and 7 non-neoplastic samples) by quantification of Gal-3 gene expression by real-time quantitative PCR (rt-PCR). Higher expression of Gal-3 was observed in GBMs and PILOs than in OD, AODs and ASTs. The IHC expression of Gal-3 was evaluated in 90 specimens (fifteen PlLOs, fourteen ASTs, 10 anaplastic astrocytomas, fifteen GBMs, eleven ODs, fifteen AODs, and 10 dysembryoplastic neuroepithelial tumors). The mean labeling score for Gal-3 determined according to the percentage of labeled cells in the tumor bulk was significantly different in GBMs versus AODs and in PILOs versus ASTs. Hence, Gal-3 is differentially expressed in central nervous system tumors, making IHC detection of Gal-3 a useful tool in distinguishing between these gliomas.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
Oct
|
| pubmed:issn |
1015-6305
|
| pubmed:author |
pubmed-author:AguiarPaulo HenriquePH,
pubmed-author:CarlottiCarlos GilbertoCGJr,
pubmed-author:ChammasRogerR,
pubmed-author:ColliBenedicto OscarBO,
pubmed-author:GabbaiAlberto AlainAA,
pubmed-author:MalheirosSuzana M FSM,
pubmed-author:MarieSuely K NagahashiSK,
pubmed-author:MiuraFlavioF,
pubmed-author:NederLucianoL,
pubmed-author:Oba-ShinjoSueli MiekoSM,
pubmed-author:RosembergSérgioS,
pubmed-author:SilvaWilson AraujoWAJr,
pubmed-author:SiqueiraRodrigo ProtoRP,
pubmed-author:UnoMiyukiM,
pubmed-author:ZagoMarco AMA
|
| pubmed:issnType |
Print
|
| pubmed:volume |
14
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
399-405
|
| pubmed:dateRevised |
2006-11-15
|
| pubmed:meshHeading |
pubmed-meshheading:15605987-Adolescent,
pubmed-meshheading:15605987-Adult,
pubmed-meshheading:15605987-Astrocytoma,
pubmed-meshheading:15605987-Brain Neoplasms,
pubmed-meshheading:15605987-Child,
pubmed-meshheading:15605987-Child, Preschool,
pubmed-meshheading:15605987-Female,
pubmed-meshheading:15605987-Galectin 3,
pubmed-meshheading:15605987-Gene Expression Regulation, Neoplastic,
pubmed-meshheading:15605987-Glioblastoma,
pubmed-meshheading:15605987-Humans,
pubmed-meshheading:15605987-Immunohistochemistry,
pubmed-meshheading:15605987-Male,
pubmed-meshheading:15605987-Middle Aged,
pubmed-meshheading:15605987-Oligodendroglioma,
pubmed-meshheading:15605987-RNA, Messenger,
pubmed-meshheading:15605987-Reverse Transcriptase Polymerase Chain Reaction,
pubmed-meshheading:15605987-Staining and Labeling
|
| pubmed:year |
2004
|
| pubmed:articleTitle |
Galectin-3 as an immunohistochemical tool to distinguish pilocytic astrocytomas from diffuse astrocytomas, and glioblastomas from anaplastic oligodendrogliomas.
|
| pubmed:affiliation |
Department of Pathology, Faculty of Medicine of Ribeirão Preto, University of São Paulo, Brazil.
|
| pubmed:publicationType |
Journal Article,
Comparative Study,
Research Support, Non-U.S. Gov't
|