Linked Life Data

15602500

Source:http://linkedlifedata.com/resource/pubmed/id/15602500

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
4
pubmed:dateCreated
2005-3-18
pubmed:abstractText
The bed nucleus of the stria terminalis (BNST) and its adrenergic input are key components in stress-induced reinstatement and maintenance of drug use. Intra-BNST injections of either beta-adrenergic receptor (beta-AR) antagonists or alpha2-adrenergic receptor (alpha2-AR) agonists can inhibit footshock-induced reinstatement and maintenance of cocaine- and morphine-seeking. Using electrophysiological recording methods in an in vitro slice preparation from C57/Bl6j adult male mouse BNST, we have examined the effects of adrenergic receptor activation on excitatory synaptic transmission in the lateral dorsal supracommissural BNST (dBNST) and subcommissural BNST (vBNST). Alpha2-AR activation via UK-14,304 (10 microM) results in a decrease in excitatory transmission in both dBNST and vBNST, an effect predominantly dependent upon the alpha2A-AR subtype. Beta-AR activation via isoproterenol (1 microM) results in an increase in excitatory transmission in dBNST, but not in vBNST. Consistent with the work with receptor subtype specific agonists, application of the endogenous ligand norepinephrine (NE, 100 microM) elicits two distinct effects on glutamatergic transmission. In dBNST, NE elicits an increase in transmission (62% of dBNST NE experiments) or a decrease in transmission (38% of dBNST NE experiments). In vBNST, NE elicits a decrease in transmission in 100% of the experiments. In dBNST, the NE-induced increase in synaptic transmission is blocked by beta1/beta2- and beta2-, but not beta1-specific antagonists. In addition, this increase is also reduced by the alpha2-AR antagonist yohimbine and is absent in the alpha2A-AR knockout mouse. In vBNST, the NE-induced decrease in synaptic transmission is markedly reduced in the alpha2A-AR knockout mouse. Further experiments demonstrate that the actions of NE on glutamatergic transmission can be correlated with beta-AR function.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Apr
pubmed:issn
0893-133X
pubmed:author
pubmed:issnType
Print
pubmed:volume
30
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
657-68
pubmed:dateRevised
2011-5-18
pubmed:meshHeading
pubmed-meshheading:15602500-Adrenergic alpha-2 Receptor Agonists, pubmed-meshheading:15602500-Adrenergic alpha-Agonists, pubmed-meshheading:15602500-Adrenergic alpha-Antagonists, pubmed-meshheading:15602500-Adrenergic beta-Agonists, pubmed-meshheading:15602500-Adrenergic beta-Antagonists, pubmed-meshheading:15602500-Animals, pubmed-meshheading:15602500-Excitatory Postsynaptic Potentials, pubmed-meshheading:15602500-Glutamic Acid, pubmed-meshheading:15602500-Male, pubmed-meshheading:15602500-Mice, pubmed-meshheading:15602500-Mice, Inbred C57BL, pubmed-meshheading:15602500-Mice, Knockout, pubmed-meshheading:15602500-Neural Inhibition, pubmed-meshheading:15602500-Norepinephrine, pubmed-meshheading:15602500-Organ Culture Techniques, pubmed-meshheading:15602500-Presynaptic Terminals, pubmed-meshheading:15602500-Receptors, Adrenergic, alpha-2, pubmed-meshheading:15602500-Septal Nuclei, pubmed-meshheading:15602500-Stress, Physiological, pubmed-meshheading:15602500-Substance-Related Disorders, pubmed-meshheading:15602500-Synaptic Transmission
pubmed:year
2005
pubmed:articleTitle
Norepinephrine modulates glutamatergic transmission in the bed nucleus of the stria terminalis.
pubmed:affiliation
Department of Molecular Physiology and Biophysics, Vanderbilt University School of Medicine, Nashville, TN 37232-0615, USA.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't, Research Support, N.I.H., Extramural