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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
8
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pubmed:dateCreated |
1992-5-13
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pubmed:abstractText |
The T cell independence of complex polysaccharide Ag has suggested the possibility that carbohydrates may be incapable of T cell recognition because of a failure to interact with MHC restriction elements and/or a failure of MHC/carbohydrate complexes to interact with and be recognized by Ag-specific TCR. We have used two approaches to obtain information about T cell recognition of carbohydrate. First, we have determined the capacity of a series of oligosaccharides and glycolipids to bind a murine class II MHC molecule, IAd. No significant binding was observed with the 26 compounds tested, but the limitation to these studies was that there was a relatively limited collection of synthetic carbohydrate and glycolipid structures of limited complexity available for analysis. The second approach involved the study of the effect of glycosylation of a known peptide T cell epitope (OVA 323-339) on MHC binding of the peptide and on T cell recognition. Three patterns of effects were observed: 1) no effect on either binding or T cell recognition. This pattern was observed when the carbohydrate was located at residues removed from the core MHC-binding region. When the carbohydrate was located within the core MHC-binding regions, either 2) glycosylation destroyed both MHC binding and T cell recognition; or 3) glycosylation did not ablate MHC binding or T cell recognition. In this latter instance, there was evidence to indicate that the carbohydrate moiety was an important part of the antigenic determinant recognized by T cells.
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pubmed:grant | |
pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
AIM
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pubmed:chemical | |
pubmed:status |
MEDLINE
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pubmed:month |
Apr
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pubmed:issn |
0022-1767
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:day |
15
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pubmed:volume |
148
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
2446-51
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pubmed:dateRevised |
2007-11-14
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pubmed:meshHeading |
pubmed-meshheading:1560200-Animals,
pubmed-meshheading:1560200-Carbohydrates,
pubmed-meshheading:1560200-Cross Reactions,
pubmed-meshheading:1560200-Female,
pubmed-meshheading:1560200-Glycopeptides,
pubmed-meshheading:1560200-Glycosylation,
pubmed-meshheading:1560200-Histocompatibility Antigens Class II,
pubmed-meshheading:1560200-Mice,
pubmed-meshheading:1560200-Mice, Inbred BALB C,
pubmed-meshheading:1560200-Peptide Fragments,
pubmed-meshheading:1560200-Structure-Activity Relationship,
pubmed-meshheading:1560200-T-Lymphocytes
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pubmed:year |
1992
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pubmed:articleTitle |
MHC interaction and T cell recognition of carbohydrates and glycopeptides.
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pubmed:affiliation |
Cytel, San Diego, CA 92121.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.
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