Source:http://linkedlifedata.com/resource/pubmed/id/15600207
Switch to
Predicate | Object |
---|---|
rdf:type | |
lifeskim:mentions | |
pubmed:issue |
1
|
pubmed:dateCreated |
2004-12-16
|
pubmed:abstractText |
OBJECTIVE: To produce high quality factor IX complex concentrates with domestic ion exchanger. A human solvent-detergent (S/D) treated factor IX complex concentrates has been produced from fresh-frozen plasma using domestic DEAE-Sepharose fast flow ion exchange chromatagraphy and Ca3(PO4)2 adsorption. The properties of this new concentrates were compared with those of the concentrates obtained by foreign DEAE-Sapharose CL-6B. RESULTS: The recovery of activity was 93.67 +/- 2.56% (n = 6) and 88.71 +/- 2 (n = 6), respectively. The specific activity of the concentrates was 7.36 +/- 0.96 U Factor IX: C/mg protein (n = 6). This new concentrates retained all the useful components with higher purity and lower thrombogenicity. Conclusion Domestic ion exchanger could replace foreign ion exchanger in producing high quality factor IX complex concentrates.
|
pubmed:language |
chi
|
pubmed:journal | |
pubmed:citationSubset |
IM
|
pubmed:chemical | |
pubmed:status |
MEDLINE
|
pubmed:month |
Jan
|
pubmed:issn |
1672-173X
|
pubmed:author | |
pubmed:issnType |
Print
|
pubmed:volume |
34
|
pubmed:owner |
NLM
|
pubmed:authorsComplete |
Y
|
pubmed:pagination |
142-4
|
pubmed:dateRevised |
2006-11-15
|
pubmed:meshHeading |
pubmed-meshheading:15600207-Chromatography, DEAE-Cellulose,
pubmed-meshheading:15600207-Detergents,
pubmed-meshheading:15600207-Drug Contamination,
pubmed-meshheading:15600207-Electrophoresis, Polyacrylamide Gel,
pubmed-meshheading:15600207-Factor IX,
pubmed-meshheading:15600207-Humans,
pubmed-meshheading:15600207-Reproducibility of Results,
pubmed-meshheading:15600207-Ultrafiltration
|
pubmed:year |
2003
|
pubmed:articleTitle |
[Technology optimization and properties study of factor IV complex concentrates].
|
pubmed:affiliation |
Department of Biopharmaceutics, West China School of Pharmacy, Sichuan University, Chengdu 610041, China.
|
pubmed:publicationType |
Journal Article,
English Abstract
|