Linked Life Data

1560017

Source:http://linkedlifedata.com/resource/pubmed/id/1560017

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
11
pubmed:dateCreated
1992-5-12
pubmed:databankReference
pubmed:abstractText
The molecular mechanisms that regulate intestine-specific gene expression and the transition from proliferating, undifferentiated crypt cells to nonproliferating, differentiated villus cells are unknown. Sucrase-isomaltase is an apical membrane disaccharidase that is found exclusively in enterocytes of adult intestine and is expressed in a complex pattern along the intestinal crypt-villus axis. To investigate the regulation of sucrase-isomaltase, we have cloned and sequenced 3.6 kilobases of the 5'-flanking region of the human sucrase-isomaltase gene. The transcriptional start site was mapped in human small intestine and in a colonic adenocarcinoma cell line (Caco-2) using an anchored polymerase chain reaction, primer extension, and RNase protection assays. The 5'-flanking DNA of the gene was linked to either chloramphenicol acetyltransferase or luciferase reporter genes and used for transfection into Caco-2, HeLa, and HepG2 cells. This analysis demonstrated that intestine-specific transcription of the sucrase-isomaltase gene involves both proximal and distal regulatory elements. Use of sucrase-isomaltase as a model gene will allow investigation of the mechanisms that regulate transcription of enterocyte-specific genes, developmental gene expression in the small intestine and colon, and the process of differentiation as epithelial cells migrate from intestinal crypts onto the villus in adult intestine.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Apr
pubmed:issn
0021-9258
pubmed:author
pubmed:issnType
Print
pubmed:day
15
pubmed:volume
267
pubmed:geneSymbol
SI
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
7863-70
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed-meshheading:1560017-Adenocarcinoma, pubmed-meshheading:1560017-Base Sequence, pubmed-meshheading:1560017-Cell Line, pubmed-meshheading:1560017-Chloramphenicol O-Acetyltransferase, pubmed-meshheading:1560017-Cloning, Molecular, pubmed-meshheading:1560017-Colonic Neoplasms, pubmed-meshheading:1560017-DNA, pubmed-meshheading:1560017-Gene Expression Regulation, Enzymologic, pubmed-meshheading:1560017-HeLa Cells, pubmed-meshheading:1560017-Humans, pubmed-meshheading:1560017-Intestine, Small, pubmed-meshheading:1560017-Luciferases, pubmed-meshheading:1560017-Molecular Sequence Data, pubmed-meshheading:1560017-Polymerase Chain Reaction, pubmed-meshheading:1560017-Regulatory Sequences, Nucleic Acid, pubmed-meshheading:1560017-Restriction Mapping, pubmed-meshheading:1560017-Sucrase-Isomaltase Complex, pubmed-meshheading:1560017-Transcription, Genetic, pubmed-meshheading:1560017-Transfection, pubmed-meshheading:1560017-Tumor Cells, Cultured
pubmed:year
1992
pubmed:articleTitle
Isolation and characterization of the human sucrase-isomaltase gene and demonstration of intestine-specific transcriptional elements.
pubmed:affiliation
Department of Internal Medicine, University of Michigan Medical School, Ann Arbor.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, U.S. Gov't, Non-P.H.S., Research Support, Non-U.S. Gov't