Source:http://linkedlifedata.com/resource/pubmed/id/15597886
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
23
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| pubmed:dateCreated |
2004-12-15
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| pubmed:abstractText |
The connection between the mode of toxic action and the genetic response caused by the type I photosensitizer and photosynthesis inhibitor neutral red (NR) and the type II photosensitizer rose bengal (RB) was investigated in the green alga Chlamydomonas reinhardtii. For both photosensitizers, a light intensity-dependent increase in toxicity and expression of the glutathione peroxidase homologous gene (Gpxh) was found. The toxicity of RB was reduced by the singlet oxygen (1O2) quenchers 1,4-diazabicyclo[2.2.2]octane and L-histidine, and the RB-induced Gpxh expression was stimulated in deuterium oxide-supplemented growth medium. These observations clearly indicate the involvement of 1O2 in both toxicity and the genetic response caused by RB. NR up-regulated the expression of typical oxidative and general stress response genes, probably by a type I mechanism, and also strongly induced the Gpxh expression. The stimulating effect of deuterium oxide in the growth medium suggested the involvement of 1O2 also in the NR-induced response. Indeed, an increased 1O2 formation was detected with EPR-spin trapping in NR-treated spinach thylakoids. However, none of the 102 quenchers could reduce the light-dependent toxicity of NR in C. reinhardtii, indicating that NR has a different mode of toxic action than RB.
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| pubmed:language |
eng
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| pubmed:journal | |
| pubmed:citationSubset |
IM
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| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Glutathione Peroxidase,
http://linkedlifedata.com/resource/pubmed/chemical/Histidine,
http://linkedlifedata.com/resource/pubmed/chemical/Neutral Red,
http://linkedlifedata.com/resource/pubmed/chemical/Photosensitizing Agents,
http://linkedlifedata.com/resource/pubmed/chemical/Piperazines,
http://linkedlifedata.com/resource/pubmed/chemical/Rose Bengal,
http://linkedlifedata.com/resource/pubmed/chemical/Singlet Oxygen,
http://linkedlifedata.com/resource/pubmed/chemical/triethylenediamine
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| pubmed:status |
MEDLINE
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| pubmed:month |
Dec
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| pubmed:issn |
0013-936X
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| pubmed:author | |
| pubmed:issnType |
Print
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| pubmed:day |
1
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| pubmed:volume |
38
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| pubmed:owner |
NLM
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| pubmed:authorsComplete |
Y
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| pubmed:pagination |
6307-13
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| pubmed:dateRevised |
2008-11-21
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| pubmed:meshHeading |
pubmed-meshheading:15597886-Animals,
pubmed-meshheading:15597886-Chlamydomonas reinhardtii,
pubmed-meshheading:15597886-Dose-Response Relationship, Drug,
pubmed-meshheading:15597886-Dose-Response Relationship, Radiation,
pubmed-meshheading:15597886-Gene Expression Regulation,
pubmed-meshheading:15597886-Glutathione Peroxidase,
pubmed-meshheading:15597886-Histidine,
pubmed-meshheading:15597886-Light,
pubmed-meshheading:15597886-Neutral Red,
pubmed-meshheading:15597886-Oxidative Stress,
pubmed-meshheading:15597886-Photosensitizing Agents,
pubmed-meshheading:15597886-Piperazines,
pubmed-meshheading:15597886-Rose Bengal,
pubmed-meshheading:15597886-Singlet Oxygen,
pubmed-meshheading:15597886-Spinacia oleracea,
pubmed-meshheading:15597886-Stress, Physiological,
pubmed-meshheading:15597886-Thylakoids,
pubmed-meshheading:15597886-Up-Regulation
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| pubmed:year |
2004
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| pubmed:articleTitle |
Photosensitizers neutral red (type I) and rose bengal (type II) cause light-dependent toxicity in Chlamydomonas reinhardtii and induce the Gpxh gene via increased singlet oxygen formation.
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| pubmed:affiliation |
Department of Environmental Microbiology and Molecular Ecotoxicology, Swiss Federal Institute for Environmental Science and Technology (EAWAG), Ueberlandstrasse 133, 8600 Dübendorf, Switzerland.
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| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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