Linked Life Data

15595737

Source:http://linkedlifedata.com/resource/pubmed/id/15595737

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
6
pubmed:dateCreated
2004-12-14
pubmed:abstractText
A modified form of the cationic colloidal silica technique for plasma membrane isolation has been combined with SDS-PAGE, mass spectrometry, and bioinformatics for evaluation as a proteomics strategy with human multiple myeloma cells and human breast cancer cells. On the basis of Western blots, half of the protein isolated is estimated to come from the plasma membrane. Forty-three percent of the 366 proteins identified by mass spectrometry had been previously classified as plasma membrane proteins. Thirty proteins previously categorized as hypothetical membrane proteins are now reported to be expressed.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:issn
1535-3893
pubmed:author
pubmed:issnType
Print
pubmed:volume
3
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1267-77
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:articleTitle
Integration of Jacobson's pellicle method into proteomic strategies for plasma membrane proteins.
pubmed:affiliation
Department of Chemistry and Biochemistry, University of Maryland, College Park, MD, USA.
pubmed:publicationType
Journal Article