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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
1
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pubmed:dateCreated |
2005-1-3
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pubmed:abstractText |
Naive T cells do not proliferate but remain alive in vivo. In contrast, naive T cells rapidly die in an in vitro culture, suggesting that some factors that are present at the sites of naive T cell circulation in vivo but missing in the bovine serum-containing culture medium, are necessary for their survival. The present study was designed to search for such factors. By functional screening of the cDNA library from murine lymph node-derived stromal cells (LNS) that effectively support the survival of naive T cells, we found that nascent polypeptide-associated complex (alpha-NAC) promoted T cell survival. A conditioned medium derived from culture supernatant of Cos7 cells transfected with alpha-NAC gene supported T cell survival, indicating that alpha-NAC induced production of soluble factor(s) that were secreted into the medium. By examining the products that were cloned from a functional screening of the cDNA library from alpha-NAC-transfected NIH3T3 cells but were not detected in that from control vector-transfected cells, galectin-1 was found as a soluble factor in the conditioned medium of the LNS. Our study demonstrates the novel role of galectin-1 as a soluble factor that functions to maintain naive T cell survival without inducing cell proliferation.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Bcl2l1 protein, mouse,
http://linkedlifedata.com/resource/pubmed/chemical/Culture Media, Conditioned,
http://linkedlifedata.com/resource/pubmed/chemical/DNA, Complementary,
http://linkedlifedata.com/resource/pubmed/chemical/Galectin 1,
http://linkedlifedata.com/resource/pubmed/chemical/Molecular Chaperones,
http://linkedlifedata.com/resource/pubmed/chemical/Proto-Oncogene Proteins c-bcl-2,
http://linkedlifedata.com/resource/pubmed/chemical/Recombinant Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Trans-Activators,
http://linkedlifedata.com/resource/pubmed/chemical/bcl-X Protein,
http://linkedlifedata.com/resource/pubmed/chemical/nascent-polypeptide-associated...
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pubmed:status |
MEDLINE
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pubmed:month |
Jan
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pubmed:issn |
0014-2980
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
35
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
86-97
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:15593120-Animals,
pubmed-meshheading:15593120-Apoptosis,
pubmed-meshheading:15593120-Base Sequence,
pubmed-meshheading:15593120-COS Cells,
pubmed-meshheading:15593120-Cattle,
pubmed-meshheading:15593120-Cell Proliferation,
pubmed-meshheading:15593120-Cell Survival,
pubmed-meshheading:15593120-Culture Media, Conditioned,
pubmed-meshheading:15593120-DNA, Complementary,
pubmed-meshheading:15593120-Galectin 1,
pubmed-meshheading:15593120-Lymphocyte Activation,
pubmed-meshheading:15593120-Mice,
pubmed-meshheading:15593120-Molecular Chaperones,
pubmed-meshheading:15593120-NIH 3T3 Cells,
pubmed-meshheading:15593120-Proto-Oncogene Proteins c-bcl-2,
pubmed-meshheading:15593120-Recombinant Proteins,
pubmed-meshheading:15593120-T-Lymphocytes,
pubmed-meshheading:15593120-Trans-Activators,
pubmed-meshheading:15593120-Transfection,
pubmed-meshheading:15593120-bcl-X Protein
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pubmed:year |
2005
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pubmed:articleTitle |
Galectin-1 supports survival of naive T cells without promoting cell proliferation.
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pubmed:affiliation |
Department of Immunology, Nagoya University Graduate School of Medicine, Nagoya 466-8550, Japan.
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pubmed:publicationType |
Journal Article,
In Vitro,
Research Support, Non-U.S. Gov't
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