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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
6
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pubmed:dateCreated |
2004-12-13
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pubmed:abstractText |
Oridonin, an extract from the Chinese herb Rabdosia rubescens, is currently one of the most important traditional Chinese herbal medicines. We investigated the anti-proliferative effect of oridonin on the lung cancer cell line SPC-A-1 and its mechanism of action. Growth inhibition was measured using a microculture tetrazolium assay and apoptosis was measured by several standard methods. Western blot analysis measured the expression of bcl-2 and bax proteins. Oridonin (> 28 micromol/l) inhibited the growth of SPC-A-1 cells and induced apoptosis. Marked morphological changes indicative of apoptosis were observed, especially in cells treated with oridonin for 48 - 60 h. Western blot analysis revealed downregulation of bcl-2 and upregulation of bax proteins following treatment with oridonin for 48 h. We conclude that oridonin demonstrated anti-proliferative and apoptosis-inducing effects on SPC-A-1 cells in vitro, and that changes in bcl-2 and bax protein levels may play an important role in its mechanism of action.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/BAX protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Bisbenzimidazole,
http://linkedlifedata.com/resource/pubmed/chemical/Coloring Agents,
http://linkedlifedata.com/resource/pubmed/chemical/Diterpenes,
http://linkedlifedata.com/resource/pubmed/chemical/Diterpenes, Kaurane,
http://linkedlifedata.com/resource/pubmed/chemical/Plant Extracts,
http://linkedlifedata.com/resource/pubmed/chemical/Proto-Oncogene Proteins c-bcl-2,
http://linkedlifedata.com/resource/pubmed/chemical/Tetrazolium Salts,
http://linkedlifedata.com/resource/pubmed/chemical/Thiazoles,
http://linkedlifedata.com/resource/pubmed/chemical/bcl-2-Associated X Protein,
http://linkedlifedata.com/resource/pubmed/chemical/oridonin,
http://linkedlifedata.com/resource/pubmed/chemical/thiazolyl blue
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pubmed:status |
MEDLINE
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pubmed:issn |
0300-0605
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
32
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
617-25
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pubmed:dateRevised |
2008-11-21
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pubmed:meshHeading |
pubmed-meshheading:15587755-Apoptosis,
pubmed-meshheading:15587755-Bisbenzimidazole,
pubmed-meshheading:15587755-Blotting, Western,
pubmed-meshheading:15587755-Cell Line, Tumor,
pubmed-meshheading:15587755-Coloring Agents,
pubmed-meshheading:15587755-DNA Fragmentation,
pubmed-meshheading:15587755-Diterpenes,
pubmed-meshheading:15587755-Diterpenes, Kaurane,
pubmed-meshheading:15587755-Dose-Response Relationship, Drug,
pubmed-meshheading:15587755-Down-Regulation,
pubmed-meshheading:15587755-Electrophoresis, Agar Gel,
pubmed-meshheading:15587755-Flow Cytometry,
pubmed-meshheading:15587755-Humans,
pubmed-meshheading:15587755-Microscopy, Electron,
pubmed-meshheading:15587755-Plant Extracts,
pubmed-meshheading:15587755-Proto-Oncogene Proteins c-bcl-2,
pubmed-meshheading:15587755-Rabdosia,
pubmed-meshheading:15587755-Tetrazolium Salts,
pubmed-meshheading:15587755-Thiazoles,
pubmed-meshheading:15587755-Time Factors,
pubmed-meshheading:15587755-Up-Regulation,
pubmed-meshheading:15587755-bcl-2-Associated X Protein
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pubmed:articleTitle |
Anti-proliferative effects of oridonin on SPC-A-1 cells and its mechanism of action.
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pubmed:affiliation |
Department of Haematology and Oncology, The Third Affiliated Hospital of Sun Yat-sen University, Guangzhou, China.
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pubmed:publicationType |
Journal Article
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