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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
Pt 12 Pt 2
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pubmed:dateCreated |
2004-12-7
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pubmed:abstractText |
Crystals of the EmrE membrane-protein imposed several technical challenges for X-ray crystallography, including high mosaicity, poor diffraction and a relatively large number of heavy atoms. Consequently, the heavy-atom substructure solution was difficult to obtain. By removing the histidine tag for protein purification, the mosaicity and the diffraction quality were greatly improved. The direct-methods Shake-and-Bake program SnB was successful in locating the heavy-atom sites from a mutant of EmrE which lacks a cysteine and therefore has a reduction in the number of heavy-atom sites. The substructure solution was solved from data with anomalous difference at a resolution of 5.5 A and the structure was determined to 3.8 A.
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pubmed:grant |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Dec
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pubmed:issn |
0907-4449
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
60
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
2399-402
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pubmed:dateRevised |
2007-11-14
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pubmed:meshHeading |
pubmed-meshheading:15583400-Antiporters,
pubmed-meshheading:15583400-Crystallography, X-Ray,
pubmed-meshheading:15583400-Cysteine,
pubmed-meshheading:15583400-Drug Resistance, Multiple,
pubmed-meshheading:15583400-Escherichia coli,
pubmed-meshheading:15583400-Escherichia coli Proteins,
pubmed-meshheading:15583400-Histidine,
pubmed-meshheading:15583400-Membrane Proteins,
pubmed-meshheading:15583400-Models, Molecular,
pubmed-meshheading:15583400-Mutation,
pubmed-meshheading:15583400-Polymerase Chain Reaction,
pubmed-meshheading:15583400-Protein Conformation
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pubmed:year |
2004
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pubmed:articleTitle |
Crystallography of the integral membrane protein EmrE from Escherichia coli.
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pubmed:affiliation |
Department of Molecular Biology, The Scripps Research Institute, 10550 North Torrey Pines Road, CB-105, La Jolla, CA 92037, USA.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, U.S. Gov't, Non-P.H.S.,
Research Support, Non-U.S. Gov't
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