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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
12
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pubmed:dateCreated |
2004-12-7
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pubmed:abstractText |
We developed a rapid PCR method utilizing the diversity of the insertion site IS1203 for genotyping Shiga toxin-producing Escherichia coli (STEC) O157 (IS1203 PCR typing). DNA fragments digested by PvuII, which cut IS1203 at one site, were ligated with themselves and detected by PCR with outward-facing primer pairs for IS1203. To minimize nonspecific bands, nested PCR was also performed. Two fingerprinting patterns produced from the upstream or downstream regions of IS1203 were obtained within 1 or 2 days. By combining the two patterns, 79 STEC O157 isolates were classified into 39 types, which were then classified into 36 subtypes by pulsed-field gel electrophoresis (PFGE). The discriminatory power of IS1203 PCR typing (D = 0.974) is similar to that of PFGE (D = 0.981). This method can be used for rapid and simplified genotyping.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/15583266-10325304,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15583266-11429469,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15583266-15109720,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15583266-16232431,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15583266-1677943,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15583266-2824781,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15583266-2832386,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15583266-3069867,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15583266-7494007,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15583266-7618896,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15583266-7883892,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15583266-7959065,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15583266-8381814,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15583266-8968924,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15583266-9196172,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15583266-9574672,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15583266-9628576
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Dec
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pubmed:issn |
0095-1137
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
42
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
5462-6
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:15583266-Animals,
pubmed-meshheading:15583266-Cattle,
pubmed-meshheading:15583266-Cattle Diseases,
pubmed-meshheading:15583266-DNA Transposable Elements,
pubmed-meshheading:15583266-Electrophoresis, Gel, Pulsed-Field,
pubmed-meshheading:15583266-Escherichia coli Infections,
pubmed-meshheading:15583266-Escherichia coli O157,
pubmed-meshheading:15583266-Genotype,
pubmed-meshheading:15583266-Humans,
pubmed-meshheading:15583266-Polymerase Chain Reaction,
pubmed-meshheading:15583266-Shiga Toxins,
pubmed-meshheading:15583266-Time Factors
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pubmed:year |
2004
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pubmed:articleTitle |
Development of a rapid PCR method using the insertion sequence IS1203 for genotyping Shiga toxin-producing Escherichia coli O157.
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pubmed:affiliation |
Department of Microbiology, Aichi Prefectural Institute of Public Health, Nagoya, Aichi, Japan. masahiro_4_suzuki@pref.aichi.lg.jp
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't,
Evaluation Studies
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