Linked Life Data

15582563

Source:http://linkedlifedata.com/resource/pubmed/id/15582563

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
2004-12-7
pubmed:abstractText
Capillary isoelectric focusing (CIEF) with liquid-core waveguide (LCW) laser-induced fluorescence (LIF) whole-column imaging detection (WCID) is a recently developed high-resolution, high-sensitivity, and high-speed analytical tool for protein analysis. Several potential applications of this system were demonstrated in this study. First, this system was employed to separate naturally fluorescent phycobiliproteins. Second, denaturing CIEF was suggested to study the conformational and chemical microheterogeneity and to characterize proteins with identical pI values. Third, a modified noncovalent fluorescent labeling procedure was presented, which allows the simple and effective labeling of proteins, antibodies, and viruses with reduced multiple labeling and preserved activity. Finally, extracellular proteins were suggested as signaling biomarkers for evaluation of cell viability. The separation of cyanobacteria and their extracellular phycoerythrins was demonstrated. The effectiveness of CIEF-LCW-LIF-WCID for the analysis of proteins, antibodies, viruses, and cells has been illustrated.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jan
pubmed:issn
0003-2697
pubmed:author
pubmed:issnType
Print
pubmed:day
1
pubmed:volume
336
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
94-101
pubmed:dateRevised
2007-11-15
pubmed:meshHeading
pubmed:year
2005
pubmed:articleTitle
Applications of capillary isoelectric focusing with liquid-core waveguide laser-induced fluorescence whole-column imaging detection.
pubmed:affiliation
Department of Chemistry, University of Waterloo, Waterloo, Ont., Canada N2L 3G1.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't